| 应用花药培养提纯粳稻不育系XG111A和保持系XG111B |
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| 引用本文： | 胡婷婷,刘 超,王健康,丁成伟,郭荣良,吴玉玲,徐家安,王友霜.应用花药培养提纯粳稻不育系XG111A和保持系XG111B[J].西北农业学报,2011,20(10):53-57. |
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| 作者姓名： | 胡婷婷 刘 超 王健康 丁成伟 郭荣良 吴玉玲 徐家安 王友霜 |
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| 作者单位： | 江苏徐淮地区徐州农业科学研究所; |
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| 基金项目： | 徐州市农业科学院科技创新基金(200804); 江苏省科技支撑计划项目(BE2009301-1); 国家科技支撑计划子课题(2006BAD01A01-7) |
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| 摘 要： | 应用花药培养技术对粳稻BT型不育系XG111A和保持系XG111B进行提纯改良。结果表明,低温6～8℃预处理3～18 d,有助于花药愈伤诱导率的提高,尤以低温预处理8 d,愈伤诱导率最高;35～50 d诱导愈伤的分化率最高,XG111A和XG111B的分化率分别达到22.5%和30.2%。经花药培养后,不育系和保持系株系内整齐度均得到显著提高:不育系的不育花粉率为100%,原供体对照为99.58%。保持系的花培后代植株90%以上都具有原供体植株的典型特征,且后代的性状遗传稳定,另有部分株系优于原供体植株。
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| 关 键 词： | 粳稻 花药培养 提纯 不育系XG111A 保持系XG111B |
Purification and Rejuvenation of CMS Lines XG111A and Maintainer Lines XG111B through Anther Culture |
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| HU Tingting,LIU Chao,WANG Jiankang,DING Chengwei,GUO Rongliang,WU Yuling,XU Jiaan and WANG Youshuang.Purification and Rejuvenation of CMS Lines XG111A and Maintainer Lines XG111B through Anther Culture[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(10):53-57. |
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| Authors: | HU Tingting LIU Chao WANG Jiankang DING Chengwei GUO Rongliang WU Yuling XU Jiaan and WANG Youshuang |
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| Institution: | HU Tingting,LIU Chao,WANG Jiankang,DING Chengwei,GUO Rongliang,WU Yuling,XU Jiaan and WANG Youshuang(Xuzhou Institute of Agricultural and Sciences,Xuzhou Jiangsu 221121,China) |
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| Abstract: | Application of anther culture in purification and rejuvenation of CMS lines XG111A and maintainer lines XG111B in rice,the results showed that: it was contributed to the improvement of anther callus induction rate that pretreated for 3-18 d at low temperature of 6-8 ℃.Especially,callus induction rate was highest for 8 d at 6-8 ℃;Differentiation rate of callus induced between 35 d and 50 d was highest for XG111A and XG111B,22.5% and 30.2% respectively;sterile rate of pollen in CMS lines of anther culture was 100%;99.58% in the field.The uniformity in lines of CMS and maintainer had been significantly improved through anther culture.About 90% normal plants obtained by anther culture showed genetic stability and some plants are superior to donor plants. |
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| Keywords: | Japonica Anther culture Purification and rejuvenation CMS lines XG111A Maintainer lines XG111B |
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