| 牛病毒性腹泻病毒石河子株E2基因的克隆及其表达载体的构建 |
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| 引用本文： | 黄新,钟发刚,薄新文,杨华,郭燕,刘志强,赵军民,任耀军,罗盘棋,王新华.牛病毒性腹泻病毒石河子株E2基因的克隆及其表达载体的构建[J].西北农业学报,2008,17(5):1-5. |
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| 作者姓名： | 黄新 钟发刚 薄新文 杨华 郭燕 刘志强 赵军民 任耀军 罗盘棋 王新华 |
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| 作者单位： | 1. 新疆兵团绵羊繁育生物技术重点实验室,新疆石河子,832000;石河子大学动物科技学院,新疆石河子,832003
2. 新疆兵团绵羊繁育生物技术重点实验室,新疆石河子,832000 |
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| 基金项目： | 兵团绵羊繁育生物技术重点实验室开放课题，兵团博士基金 |
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| 摘 要： | 应用RT-PCR方法从牛病毒性腹泻病毒(BVDV)新疆石河子分离株中扩增了E2基因,命名Shihezi 148(GenBank登录号:EU159699),扩增序列分析结果表明:Shihezi 148 E2基因长度为1121 bp,编码373个氨基酸残基;同源性分析表明,Shihezi 148与澳大利亚Bega株E2基因核苷酸同源性为88.32%,与中国changchun 184株的同源性为74.42%;通过基因重组技术构建了去除C端跨膜区的截短E2基因的原核表达载体pProEX HTa-E2和包括信号肽和全长E2蛋白的真核重组质粒pVAX1-E2。经过PCR、酶切及测序结果表明pProEX HTa-E2和pVAX1-E2重组表达载体构建成功。
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| 关 键 词： | 牛病毒性腹泻病毒 E2基因 克隆 表达载体 |
| 收稿时间： | 2008/3/3 0:00:00 |
| 修稿时间： | 2008/4/18 0:00:00 |
Cloning of the E2 Gene of Bovine Viral Diarrhea Virus Isolated of Shihezi and Construction of Expression Vector |
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| HUANG Xin,ZHONG Fa-gang,BO Xin-wen,YANG Hu,GUO Yan,LIU Zhi-qiang,ZHAO Jun-min,REN Yao-jun,LUO Pan-qi and WANG Xin-hua.Cloning of the E2 Gene of Bovine Viral Diarrhea Virus Isolated of Shihezi and Construction of Expression Vector[J].Acta Agriculturae Boreali-occidentalis Sinica,2008,17(5):1-5. |
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| Authors: | HUANG Xin ZHONG Fa-gang BO Xin-wen YANG Hu GUO Yan LIU Zhi-qiang ZHAO Jun-min REN Yao-jun LUO Pan-qi and WANG Xin-hua |
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| Institution: | Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China;College of Animal Science, Shihezi University, Shihezi Xinjiang 832003, China;Key Laboratory of Sheep Reproduction Biology of Bingtuan, Shihezi Xingjiang 832000, China |
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| Abstract: | |
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| Keywords: | Bovine viral diarrhea virus E2 gene Clone Expression vector |
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