| 黄薇花粉活力及柱头可授性 |
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| 引用本文： | 罗卿清,郑钢,顾翠花,顾帆,陈凯,郑绍宇.黄薇花粉活力及柱头可授性[J].浙江农林大学学报,2020,37(1):182-187. |
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| 作者姓名： | 罗卿清 郑钢 顾翠花 顾帆 陈凯 郑绍宇 |
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| 作者单位： | 浙江农林大学 风景园林与建筑学院, 浙江 杭州 311300 |
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| 基金项目： | 浙江省新苗人才计划项目2018R412014 |
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| 摘 要： | 目的 研究不同条件下，黄薇Heimia myrtifolia的花粉萌发及花粉管生长情况。 方法 以黄薇为材料，采用花粉离体萌发法研究不同温度、蔗糖质量浓度和硼酸质量浓度对黄薇花粉萌发及花粉管生长的影响；用联苯胺-过氧化氢法测定柱头可授性，研究不同质量浓度萘乙酸、赤霉素、6-苄基腺嘌呤对黄薇柱头可授性的影响，并对黄薇花粉与柱头进行电镜扫描观察。 结果 ① 不同温度、蔗糖质量浓度、硼酸质量浓度对黄薇花粉的萌发生长影响差异明显，培养黄薇花粉适宜温度为35℃，蔗糖适宜质量浓度为50.0~150.0 g·L-1，硼酸适宜质量浓度为0.5~1.0 g·L-1；②黄薇柱头可授性受时间影响，在开花6~8 h柱头可授性最强；③不同植物生长调节剂对黄薇柱头可授性影响差异明显，提高黄薇柱头可授性最适质量浓度为萘乙酸10.0 mg·L-1、赤霉素200.0~400.0 mg·L-1、6-苄基腺嘌呤20.0 mg·L-1；④黄薇柱头直径为425.78~546.93 μm，花粉极轴长为23.15~26.74 μm，赤道轴长为18.02~20.03 μm，属小型花粉。 结论 黄薇人工授粉的最佳时期在开花后6~8 h，结合一定质量浓度的植物生长调节剂，可以有效提高结实率。
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| 关 键 词： | 园艺学 黄薇 花粉活力 花粉管生长 柱头可授性 |
| 收稿时间： | 2018-12-11 |
Pollen viability and stigma receptivity of Heimia myrtifolia |
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| LUO Qingqing,ZHENG Gang,GU Cuihua,GU Fan,CHEN Kai,ZHENG Shaoyu.Pollen viability and stigma receptivity of Heimia myrtifolia[J].Journal of Zhejiang A&F University,2020,37(1):182-187. |
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| Authors: | LUO Qingqing ZHENG Gang GU Cuihua GU Fan CHEN Kai ZHENG Shaoyu |
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| Institution: | School of Landscape Architecture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China |
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| Abstract: | Objective To determine the effects of different temperatures, sucrose concentrations, and boric acid concentrations on pollen germination and pollen tube growth of Heimia myrtifolia, and study the effects of different concentrations of naphthylacetic acid(NAA), gibberellin (GA3), and 6-benzyladenine (6-BA) on stigma receptivity of H. myrtifolia. Method In vitro pollen germination, the agar medium germination and benzidine-hydrogen peroxide methods were used. The stigma and pollen were observed under a scanning electron microscope. Result The effects of different temperatures, sucrose concentrations, and boric acid concentrations on pollen germination and on pollen tube growth were significantly different (P < 0.05). Suitable concentrations of boric acid and sucrose on pollen germination ranged from 0.05% to 0.10% and on pollen tube growth ranged from 5% to 15%. The suitable storage temperature was 35℃. The stigma of H. myrtifolia had different receptivity during the blooming period with the highest receptivity 6-8 hours after blooming. The effects of the hormone with different concentrations of NAA, GA3, and 6-BA on pollen viability and growth of H. myrtifolia were significantly different (P < 0.05) with the optimum concentrations being 10.0 mg·L-1 NAA, 200.0-400.0 mg·L-1 GA3, and 20.0 mg·L-1 6-BA. Stigma diameter of H. myrtifolia ranged from 425.78 μm to 546.93 μm. Scanning electron microscopy of the H. myrtifolia pollen showed that the polar axis length ranged from 23.15 μm to 26.74 μm, and the equatorial axis length ranged from 18.02 μm to 20.03 μm qualifying it as a small-sized pollen. Conclusion The optimum time for artificial pollination of H. myrtifolia was 6-8 hours after anthesis, and hormone stimulation of a specific concentration could effectively improve the seed setting rate. |
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