| 番鸭硬酯酰辅酶A去饱和酶1基因克隆、序列分析及填饲对其表达的影响 |
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| 引用本文： | 王健,张军,段修军,张宜辉,张蕊,董飚,龚道清.番鸭硬酯酰辅酶A去饱和酶1基因克隆、序列分析及填饲对其表达的影响[J].上海交通大学学报(农业科学版),2014(3):39-44. |
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| 作者姓名： | 王健 张军 段修军 张宜辉 张蕊 董飚 龚道清 |
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| 作者单位： | [1] 江苏农牧科技职业学院,江苏泰州225300; [2] 扬州大学动物科学与技术学院,江苏扬州225009 |
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| 基金项目： | 江苏省“333工程”（苏人才[2011]15号）;江苏省“青蓝工程”（苏教师[2012]39号） |
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| 摘 要： | 研究旨在克隆番鸭硬酯酰辅酶A去饱和酶1(SCD1)基因,并探讨该基因组织特异性表达规律以及填饲对其表达的影响。试验以番鸭为材料,采用RT-PCR方法从番鸭肝脏中扩增出SCD1基因完整CDS区域,并采用相关分子生物学软件对所获得的基因片段进行分析,同时利用实时荧光定量PCR方法检测SCD1基因在番鸭12种组织以及填饲不同阶段肝脏和脂肪组织中mRNA表达丰度。结果表明,所扩增的SCD1基因编码完整的开放阅读框,ORF长度为1 083bp,共编码360个氨基酸;与GenBank中禽类的氨基酸同源性在91%~97%之间,与哺乳类动物同源性在80%~90%之间,与鱼类同源性在68%~75%之间。实时荧光定量PCR结果显示SCD1基因在肝脏和腹脂中表达最高,在其他组织中少量表达或未检测到表达。填饲使得番鸭肝脏组织中SCD1基因表达极显著升高,脂肪组织中SCD1基因表达量显著升高,揭示SCD1基因可能在番鸭肥肝形成中起到重要作用。
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| 关 键 词： | 番鸭 填饲 SCD1 基因克隆 组织表达 |
| 收稿时间： | 2013/7/1 0:00:00 |
Cloning and Analysis of SCD1 Gene and the Effect of Overfeeding on Its mRNA Level in Muscovy Duck |
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| WANG Jian,ZHANG Jun,DAUAN Xiu-jun,ZHANG Yi-hui,ZHANG Rui,DONG Biao and GONG Dao-qing.Cloning and Analysis of SCD1 Gene and the Effect of Overfeeding on Its mRNA Level in Muscovy Duck[J].Journal of Shanghai Jiaotong University (Agricultural Science),2014(3):39-44. |
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| Authors: | WANG Jian ZHANG Jun DAUAN Xiu-jun ZHANG Yi-hui ZHANG Rui DONG Biao and GONG Dao-qing |
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| Institution: | WANG Jian , ZHANG Jun, DAUAN Xiu-jun , ZHANG Yi-hui, ZHANG Rui, DONG Biao, GONG Dao-qing ( 1. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China; 2. College of Animal Science and Technology,Yangzhou University, Yangzhou 225009 ,China) |
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| Abstract: | The aim of this study was to clone and analyze the muscovy duck SCD1 gene, and reveal the expression of SCD1 gene mRNA in different tissues in muscovy duck and the effect of overfeeding on SCD1 mRNA level. The muscovy duck were used as the subjects to clone the sequence of SCD1 gene by RT-PCR,and the tissue-specific expression and the effect of overfeeding on SCD1 mRNA level were performed by real-time PCR. The results showed that the cloned sequence contained an open reading frame （ORF） 1 083 bp in length and encoded 360 amino acids. The amino acid homology of muscovy duck SCD1 were 91%-97% with SCD1 of other avian species,80%-90% with SCD1 of mammals,68%-75% with SCD1 of fishes. The tissue-specific expression results showed that the mRNA expression level ofmuscovy duck SCD1 gene was tissues,the mRNA expression muscovy duck SCD1 gene in indicated that SCD1 gene may high in liver and adipose tissues, and was not detected or little in other 10 of muscovy duck SCD1 gene was tissue specific. The mRNA expression of liver and adipose tissues was significantly increased after overfeeding. It play an important role in duck liver steatosis. |
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| Keywords: | muscovy duck overfeeding SCD1 gene clone tissue expression |
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