| 不同浓度甘油冷冻保护剂和冷冻麦管规格对七彩鲑受精率的影响 |
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| 引用本文： | 邓双义,李胜忠,成沛,关秀芝.不同浓度甘油冷冻保护剂和冷冻麦管规格对七彩鲑受精率的影响[J].现代农业科技,2012(1):293+296. |
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| 作者姓名： | 邓双义 李胜忠 成沛 关秀芝 |
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| 作者单位： | 新疆农业大学动物科学学院,新疆乌鲁木齐,830052 |
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| 摘 要： | 以5%～30%浓度的甘油作为精液冷冻保护剂,以0.5 mL麦管和1.8 mL麦管作为精液冻存容器,进行七彩鲑冻精的受精试验。试验结果表明:20%浓度甘油组的受精率达到了51.08%～61.56%,显著高于其他浓度甘油组的受精率;在20%～25%甘油浓度中0.5 mL麦管组的受精率均高于1.8 mL麦管组的受精率,其中20%甘油的0.5 mL麦管组,平均受精率最高,达到了61.38%。
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| 关 键 词： | 甘油 冷冻保护剂 冷冻麦管 七彩鲑 受精率 影响 |
Effects of Different Concentrations of Glycerol as Cryoprotectant and Different Sizes of Straw on Fertilization Rate of Salvelinus fontinalis |
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| Authors: | DENG Shuang-yi LI Sheng-zhong CHENG Pei GUAN Xiu-zhi |
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| Institution: | DENG Shuang-yi LI Sheng-zhong CHENG Pei GUAN Xiu-zhi(College of Animal Science,Xinjiang Agricultural University,Urumqi Xinjiang 830052) |
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| Abstract: | The artificial fertilization experiment on Salvelinus fontinalis was carried out,using 5%～30% glycerol as cryoprotectant,and 0.5 mL and 1.8 mL straw as semen cryopreservation container.The results showed that:The fertilization rates of 20% glycerol group reached 51.08%～61.56%,which was obviously higher than other concentrations of glycerol groups.With the glycerol concentration of 20%～25%,the fertilization rates of 0.5 mL straw group were significantly higher than the fertilization rates of 1.8 mL straw group.The mean fertilization rate of 20% glycerol and 0.5 mL straw group was the highest,which reached 61.38%. |
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| Keywords: | glyeerol eryoproteetant straw Salvelinusfontinalis fertilizationrate effeet |
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