| 不同动物粪源葡萄球菌耐药性调查及耐药基因检测分析 |
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| 引用本文： | 杨紫嫣,姚晓慧,马木尔·阿克木汉,王舒丰,王凯,夏利宁.不同动物粪源葡萄球菌耐药性调查及耐药基因检测分析[J].新疆农业科学,2020,57(5):967-973. |
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| 作者姓名： | 杨紫嫣 姚晓慧 马木尔·阿克木汉 王舒丰 王凯 夏利宁 |
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| 作者单位： | 新疆农业大学动物医学学院,乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金－新疆联合基金项目(U1503185) |
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| 摘 要： | 【目的】研究新疆霍城县不同动物粪源葡萄球菌对临床常用抗菌药物的耐药情况。【方法】新疆霍城县主要的规模化养殖场分别采集猪、鸡、牛及羊的粪便。采用琼脂稀释法对分离出的葡萄球菌进行最小抑菌浓度测定。通过PCR方法检测相应耐药基因。【结果】不同动物粪源葡萄球菌对被检抗菌药物耐药严重程度依次为猪粪源葡萄球菌＞鸡粪源葡萄球菌＞羊粪源葡萄球菌＞牛粪源葡萄球菌;鸡粪源葡萄球菌及猪粪源葡萄球菌对苯唑西林、四环素耐药率均超过80%。牛粪源葡萄球菌对庆大霉素和阿米卡星敏感率达100%。ermB基因是猪粪源葡萄球菌和鸡粪源葡萄球菌中检出率最高的林可胺类耐药基因,检出率分别是70.8%和93.5%;femA基因是羊粪源葡萄球菌和牛粪源葡萄球菌中检出率最高的β-内酰胺类耐药基因。【结论】新疆霍城县不同动物粪源葡萄球菌对被检抗菌药物的耐药程度不同,多药耐药情况严重,在养殖过程中应考虑耐药情况需合理使用抗菌药物。
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| 关 键 词： | 不同动物粪源 葡萄球菌 多药耐药 耐药基因 |
| 收稿时间： | 2019-09-18 |
Investigation of Drug Resistance of Different Animal Fecal Staphylococci and Detection of Drug Resistance Genes |
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| YANG Ziyan,YAO Xiaohui,MAmuer Akmuhan,WANG Shufeng,WANG Kai,XIA Lining.Investigation of Drug Resistance of Different Animal Fecal Staphylococci and Detection of Drug Resistance Genes[J].Xinjiang Agricultural Sciences,2020,57(5):967-973. |
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| Authors: | YANG Ziyan YAO Xiaohui MAmuer Akmuhan WANG Shufeng WANG Kai XIA Lining |
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| Institution: | College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | 【Objective】 To investigate the drug resistance of Staphylococcus faecalis from different animals to commonly used antibiotics in Huocheng County, Xinjiang.【Method】Feces of pigs, chickens, cattle and sheep were collected from the major scale farms in the County. , After that, the minimum inhibitory concentration of Staphylococcus isolated from the feces was determined by agar dilution method and the corresponding resistance genes were detected by PCR method. 【Result】The severity of antibiotic resistance of Staphylococcus faecalis to tested antibiotics in different animals was as follows: Staphylococcus from pigs ＞ Staphylococcus from chickens ＞ Staphylococcus from sheep ＞ Staphylococcus from cattle; The resistance rate of Staphylococcus from chickens and pigs to oxacillin tetracycline was more than 80%. The sensitivity of Staphylococcus to gentamicin and amikacin was 100%. The detection rates of Staphylococcus from chickens and pigs were the highest for the ermB gene, whose detection rates were 70.8% and 93.5%, respectively; the highest detection rates of Staphylococcus from sheep and cattle fecal were femA gene. 【Conclusion】The resistance of different animal fecal Staphylococcus to the tested antibiotics in Huocheng County of Xinjiang was different, and the multi-drug resistance was serious. In the process of breeding, the antibiotics should be rationally used. |
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| Keywords: | different animal feces Staphylococcus multidrug resistance resistance gene |
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