| 哈萨克母羊分娩前与胎儿组织器官细菌的多样性 |
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| 引用本文： | 李晓斌,马晨,李凤鸣,黄新新,米热古丽·伊马木,臧长江,李佳豪,李超,马利鑫,曹蕊.哈萨克母羊分娩前与胎儿组织器官细菌的多样性[J].新疆农业科学,2021,58(5):965-972. |
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| 作者姓名： | 李晓斌 马晨 李凤鸣 黄新新 米热古丽·伊马木 臧长江 李佳豪 李超 马利鑫 曹蕊 |
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| 作者单位： | 新疆农业大学动物科学学院,乌鲁木齐 830052 |
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| 基金项目： | 天池博士计划项目(2018XJTC0012);大学生创新项目 |
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| 摘 要： | 【目的】研究分娩前哈萨克母羊和胎儿组织、肠道内容物及相关器官菌群的多样性。【方法】试验选择5只4岁、胎次相同、健康的妊娠后期母羊,平均体重为(55.64±4.37) kg,安乐死后,分离各组织器官并采集相关样品,用于分析菌群多样性。【结果】(1)母羊唾液、小肠内容物及羊水中有较高的多样性,chao1指数分别为1 042.02、1 058.31、1 011.22,ACE指数分别为1 069.18、1 067.41、1 014.17。(2)在物种分类水平上,母羊小肠、盲肠内容物、脐带及羔羊胎粪中厚壁菌门丰度较高,占比分别为79.08%、65.94%、49.66%和35.69%;在母羊唾液和羔羊瘤胃中拟杆菌门占比较高,分别为26.98和51.80%;羔羊瘤胃和母羊唾液中变形菌门的占比分别为64.92%和41.92%。(3)羔羊瘤胃液、胎粪及脐带中物种相似性较高,母羊唾液和瘤胃液中物种相似性较高,母羊小肠内容物和母羊盲肠内容物中物种相似性较高。【结论】母羊妊娠后期唾液、小肠内容物及羊水中有较高的多样性指数,厚壁菌门、变形菌门及拟杆菌门是此阶段母羊和胎儿各组织器官主要的物种。
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| 关 键 词： | 哈萨克母羊 胎儿 菌群结构 多样性 |
| 收稿时间： | 2020-05-13 |
Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe |
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| LI Xiaobin,MA Chen,LI Fengming,HUANG Xinxin,Yimamu Mireguli,ZANG Changjiang,LI Jiahao,LI Chao,MA Lixin,CAO Rui.Study on Microbiota Diversity of Maternal and Fetus at the Late Pregnancy of Kazakh Ewe[J].Xinjiang Agricultural Sciences,2021,58(5):965-972. |
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| Authors: | LI Xiaobin MA Chen LI Fengming HUANG Xinxin Yimamu Mireguli ZANG Changjiang LI Jiahao LI Chao MA Lixin CAO Rui |
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| Institution: | College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | 【Objective】 The purpose of this experiment is to study the microbiota diversity of the flora of Kazakh ewe and fetal tissues, intestinal contents and related organs before delivery. 【Methods】 Five 4-year-old Kazakh ewe with average body weight of (55.64±4.37) kg and the same parity were selected. After euthanasia, tissue organ samples were isolated and collected and analyzed for bacterial diversity. 【Results】 (1) There were higher diversity of the saliva, smaller intestine contents and amniotic fluid in the ewes; the chao1 index were 1,042.02, 1,058.31, 1,011.22 and the ACE index were 1,069.18, 1,067.05, 1,014.17, respectively. (2)At the species classification level, the abundance of firmicutes was higher in the small intestine, cecal contents, umbilical cord and the ewe and lamb meconium, accounting for 79.08%, 65.94%, 49.66% and 35.69%, respectively. The bacteroides of saliva and lamb rumen was 26.98 and 51.80%, respectively. The proteobacteria of saliva and lamb rumen was 64.92% and 41.92%, respectively. (3)The results of aggregation showed that the similarity of the species in the rumen fluid, meconium and umbilical cord of the lamb was higher, the similarity of the species in the saliva of the ewes and the rumen fluid was higher, and the similarity of the contents of the small intestine of the ewes and the cecal contents of the ewe were higher. 【Conclusion】 Therefore, the saliva, small intestine contents and amniotic fluid of the ewes have higher diversity index in the late pregnancy, and the thick-walled bacteria, the proteobacteria and the bacteroides are the main species of the tissues and organs of the ewes and fetuses at this stage. |
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| Keywords: | Kazakh ewes fetus flora structure diversity |
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