| 枯草芽孢杆菌D-29拮抗作用及其抗菌蛋白特性研究 |
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| 引用本文： | 谈静惠,高莹,程超,李宏,王美琴,郝晓娟.枯草芽孢杆菌D-29拮抗作用及其抗菌蛋白特性研究[J].山西农业大学学报(自然科学版),2012,32(3):224-227. |
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| 作者姓名： | 谈静惠 高莹 程超 李宏 王美琴 郝晓娟 |
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| 作者单位： | 1. 山西农业大学农学院,山西太谷,030801
2. 山西农业大学生命科学学院,山西太谷,030801 |
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| 基金项目： | 国家自然基金青年基金(31000873);山西省青年基金(2010021031-1);山西农业大学博士后基金;山西农业大学科技创新基金(2009019);山西省科技攻关项目(20080311025) |
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| 摘 要： | 枯草芽孢杆菌D-29是从地肤根内分离获得的一株生防细菌,为进一步了解D-29的生防特性,采用平板对峙法测定其抑菌谱并对其抑菌蛋白进行了研究。结果表明,该菌对番茄灰霉病菌、玉米大斑病菌等11种植物病原真菌均具有抑制作用,其中对玉米大斑病菌的抑菌效果最好,抑制率为54.6%;确定80%饱和硫酸铵为最佳盐析饱和度,获得的粗提蛋白耐高温,对有机溶剂和紫外光照不敏感,耐酸不耐碱。
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| 关 键 词： | 枯草芽孢杆菌 拮抗作用 抑菌蛋白 |
Antagonistic Effect of Bacillus subtilis D-29 and Characterization of its Antifungal Protein |
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| Tan Jinghui
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Gao Ying
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Cheng Chao
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Li Hong
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Wang Meiqin
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Hao Xiaojuan.Antagonistic Effect of Bacillus subtilis D-29 and Characterization of its Antifungal Protein[J].Journal of Shanxi Agricultural University,2012,32(3):224-227. |
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| Authors: | Tan Jinghui Gao Ying Cheng Chao Li Hong Wang Meiqin Hao Xiaojuan |
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| Institution: | 1(1.College of Agriculture,Shanxi Agricultural University,Taigu Shanxi 030801,China;2.College of Life Sciences,Shanxi Agricultural University,Taigu Shanxi 030801,China) |
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| Abstract: | Biocontrol bacterial strain Bacillus subtilis D-29 was isolated from the root of Kochia scoparia.To gain valuable information for antagonistic effect,inhibition spectrum of D-29 was tested by plates inhibition methods and its antifungal protein was studied.D-29 showed antifungal activity against 11 pathogens.It showed the strongest antagonistic activity against Exserohilum turcicum and inhibitory rate reached 54.6%.Crude protein was extracted with ammonium sulphate at 80% saturation.The crude protein endured acid,heat,organic solvent and UV-irradiation,but it was sensitive to alkali. |
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| Keywords: | Bacillus subtilis Antagonistic effect Antifungal protein |
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