| 混合重金属离子对鲫鱼(Carassius aruatus) Na/KATPase活性的影响及基因毒性作用研究 |
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| 引用本文： | 周新文,朱国念,孙锦荷.混合重金属离子对鲫鱼(Carassius aruatus) Na/KATPase活性的影响及基因毒性作用研究[J].浙江大学学报(农业与生命科学版),2001,27(6):665-669. |
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| 作者姓名： | 周新文 朱国念 孙锦荷 |
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| 作者单位： | 浙江大学核农业科学研究所, |
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| 摘 要： | 研究了混合重金属对鲫鱼Na/K ATPase活性及DNA合成作用的影响,结果表明:鱼鳃、鱼脑和肝脏的Na/K ATPase活性与混合重金属的浓度呈显著的负线性相关关系;3种组织Na/K ATPase活性的敏感顺序为鱼脑>鱼鳃>肝脏.且抑制这3种组织Na/K ATPase活性50%的浓度分别为1.63mg/kg,0.92 mg/kg和2.02 mg/L.DNA掺入试验表明:注射3H-TdR后取样的最佳时间为6 h;混合重金属对鱼体各组织DNA合成作用呈现双向效应,即低浓度时,3H-TdR掺入量增大,表现出损伤、修复作用,高浓度时,3H-TdR的掺入量显著低于对照,组织细胞DNA的合成受到了抑制作用;鱼鳃和肝脏的Na/K ATPase活性与掺入组织DNA中3H-TdR的放射性有显著的相关性.
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| 关 键 词： | 混合重金属 鲫鱼 ATPase DNA |
| 文章编号： | 1008-9209(2001)06-0665-05 |
| 修稿时间： | 2001年1月26日 |
Effect of mixture metals on the fish (Carassius auratus) ATPase activity and its genotoxicity |
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| ZHOU Xin-wen,ZHU Guo-nian,SUN Jin-he.Effect of mixture metals on the fish (Carassius auratus) ATPase activity and its genotoxicity[J].Journal of Zhejiang University(Agriculture & Life Sciences),2001,27(6):665-669. |
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| Authors: | ZHOU Xin-wen ZHU Guo-nian SUN Jin-he |
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| Abstract: | The Carassius auratus genetoxicity and ATPase activity for mixture metals have been studied.The results demonstrated that there was negative linear correlation between the Na/K ATPase activity and the exposure concentration of mixture metals in the tissue of gill, brain and liver as well. The sensitivity order of the Na/K ATPase activity among the three tissues was brain>gill>liver for the mixture metal, their EC 50 was 1.63, 0.92, 2.02 mg/L respectively. The 3H-TdR incorporation experiment demonstrated that the best sampling time was 6 h after injection. The mixture-metal toxicity for Carassius auratus was double effect, the DNA synthesis was damaged at lower concentration and would be inhibited at higher concentration; There is a significant correlationship between the Na/K ATPase activity and 3H-TdR radioactivity in the tissue of liver and gill. |
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| Keywords: | mixture-metals Carassius auratus ATPase genotoxicity |
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