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| 用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定 | |
| 引用本文： | 张晓勤,薛大伟,周伟辉,邬飞波,张国平.用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定[J].浙江大学学报(农业与生命科学版),2011,37(2). |
| 作者姓名： | 张晓勤 薛大伟 周伟辉 邬飞波 张国平 |
| 作者单位： | 1. 杭州师范大学,生命与环境科学学院,浙江,杭州,310036;浙江大学,农业与生物技术学院,农学系,浙江,杭州,310029 2. 杭州师范大学,生命与环境科学学院,浙江,杭州,310036 3. 浙江大学,农业与生物技术学院,农学系,浙江,杭州,310029 |
| 基金项目： | 农业部农业公益性行业科研专项"核技术农业应用"资助项目 |
| 摘 要： | 利用化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)诱变处理大麦品种浙农大3号的种子,然后种于大田,将获得的M2代材料进行生物学性状与农艺性状鉴定,入选的M3代材料继续进行验证.结果表明,实验获得了幼苗、生育期、叶、茎秆、穗及育性等生物学特性与主要农艺性状的突变体,变异类型丰富,突变群体的表型变异率约为7.46%.本研究构建的突变群体为大麦功能基因组研究奠定了基础,同时为大麦遗传改良提供了重要的材料. |
| 关 键 词： | 大麦 甲基磺酸乙酯 突变体 群体 |
Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS) |
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| ZHANG Xiao-qin,XUE Da-wei,ZHOU Wei-hui,WU Fei-bo,ZHANG Guo-ping.Screening and identification of the mutants from two-row barley cultivar ZJU3 induced by ethyl methane sulfonate (EMS)[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(2). | |
| Authors: | ZHANG Xiao-qin XUE Da-wei ZHOU Wei-hui WU Fei-bo ZHANG Guo-ping |
| Institution: | ZHANG Xiao-qin1,2,XUE Da-wei1,ZHOU Wei-hui2,WU Fei-bo2,ZHANG Guo-ping2(1.College of Life and Environment Sciences,Hangzhou Normal University,Hangzhou 310036,China,2.Department of Agronomy,College of Agriculture & Biotechnology,Zhejiang University,Hangzhou 310029,China) |
| Abstract: | The seeds of two-row barley(Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate(EMS) to develop a mutant library.In M2 generation,biological and agronomic characters were examined,and the identified mutants were validated in M3 generation.Totally 97 phenotype mutants,involved in seedling habits,maturity,leaf morphology,plant height,spike shape and spikelet fertility were obtained from 3 000 treated plants,with mutated frequency of 7.46%.The results indicate the feasibi... |
| Keywords: | barley ethyl methane sulfonate(EMS) mutant population |
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