| 五汁饮对急性酒精中毒大鼠肝脏的保护机制研究 |
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| 作者姓名： | 谢梦洲 冯楚雄 朱建平 瞿昊宇 肖作为 向茗 吴玉冰 |
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| 作者单位： | 湖南中医药大学中医诊断研究所, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208;数字中医药协同创新中心, 湖南 长沙 410208,湖南中医药大学中医诊断研究所, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208,湖南中医药大学中医诊断研究所, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208,湖南中医药大学中医诊断研究所, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208;数字中医药协同创新中心, 湖南 长沙 410208,湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208,湖南中医药大学中医诊断研究所, 湖南 长沙 410208;湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208,湖南省药食同源功能性食品工程技术研究中心, 湖南 长沙 410208 |
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| 基金项目： | 湖南省教育厅创新平台基金资助（12k083）；湖南中医药大学中医诊断学国家重点学科开放基金资助（2014-27）。 |
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| 摘 要： | 目的 研究五汁饮对急性酒精中毒大鼠肝脏的保护作用。方法 SD大鼠分为5组，分别为空白组、模型组、葛花组、五汁饮低剂量组及高剂量组，每组10只。预防性给药 7 d后，空白组蒸馏水灌胃，其余各组按照1.5 mL/100 g的52度红星二锅头给予灌胃，每日1次，连续1周，建立急性酒精性肝损伤模型。测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）含量；测定肝脏匀浆中谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、丙二醛（MDA）、乙醇脱氢酶（ADH）含量。结果 与模型组相比，葛花组、五汁饮高剂量组ALT含量降低（P<0.05），五汁饮高、低剂量组AST含量降低（P<0.01）；五汁饮高剂量组ADH含量高于模型组（P<0.05），葛花组、五汁饮低剂量组与模型组没有统计学差异（P>0.05）；葛花组、五汁饮高、低剂量组GSH、SOD含量高于模型组（P<0.01，P<0.05），葛花组、五汁饮高、低剂量组均可显著降低MDA含量（P<0.01）。结论 五汁饮保肝作用可能通过激活ADH加快酒精代谢及SOD、GSH的抗氧化作用来实现。
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| 关 键 词： | 酒精性肝损伤 五汁饮 抗氧化 乙醇脱氢酶 |
| 收稿时间： | 2016/3/24 0:00:00 |
The Protective Mechanism of Wuzhiyin on the Liver of Rats With Acute Alcoholism |
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| Authors: | XIE Mengzhou FENG Chuxiong ZHU Jianping QU Haoyu XIAO Zuowei XIANG Ming and WU Yubing |
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| Institution: | Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China;Collaborative Innovation Center of Digital Chinese Medicine, Changsha, Hunan 410208, China,Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China,Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China,Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China;Collaborative Innovation Center of Digital Chinese Medicine, Changsha, Hunan 410208, China,Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China,Institute of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China and Hunan Engineering Research Center of Medicine and Food Homology Functional Food, Changsha, Hunan 410208, China |
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| Abstract: | Objective To study the effect of Wuzhiyin on the liver of rats with acute alcoholism. Methods SD rats were divided into 5 groups:blank group, model group, Gehua group, low dose group and high dose group of Wuzhiyin, 10 rats in each group. After 7days preventive administration, the blank group was given a gavage of distilled water and the remaining groups were treated with 1.5 mL/100 g 52% alcohol, once a day for one week, and the model of acute alcoholic liver injury was established. The content of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) were detected and the content of liver homogenate glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA), alcohol dehydrogenase (ADH) were also detected. Results Compared with the model group, the content of ALT in Gehua group and high dose Wuzhiyin group was decreased (P<0.05). The content of AST in high and low dose of Wuzhiyin group was reduced (P<0.01). The content of ADH in the high dose Wuzhiyin group was higher than the model group (P<0.05), and there was no significant difference between the model group and the low dose group of Wuzhiyin (P>0.05). The content of GSH and SOD in Gehua group, high and low dose of groups was higher than that in model group (P<0.01, P<0.05). The content of MDA in Gehua group, the high and low dose of Wuzhiyin groups was significantly decreased (P<0.01). Conclusion The hepatoprotective effect of Wuzhiyin was possibly through activation of ADH to accelerate the metabolism of alcohol and antioxidant. |
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| Keywords: | alcoholic liver injury Wuzhiyin antioxidant alcohol dehydrogenase |
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