| 猪屎豆抗氧化活性成分的提取及活性测定体系的优化 |
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| 引用本文： | 姜佩佩,纪明慧,舒火明,李渊.猪屎豆抗氧化活性成分的提取及活性测定体系的优化[J].安徽农业科学,2010,38(28):15571-15573. |
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| 作者姓名： | 姜佩佩 纪明慧 舒火明 李渊 |
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| 作者单位： | 海南师范大学,省部共建-热带药用植物化学教育部重点实验室,海南海口,571158;海南师范大学,省部共建-热带药用植物化学教育部重点实验室,海南海口,571158;琼台师范高等专科学校,海南海口,571100 |
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| 摘 要： | 目的]研究猪屎豆（Crotalaria mucronata Desv.）乙醇提取物的抗氧化活性。方法]采用超声波辅助乙醇提取法提取猪屎豆抗氧化活性成分,用H2O2-鲁米诺化学发光体系测定清除羟自由基（·OH）的效率。并对测定体系进行优化。结果]在料液比为3.6∶1,乙醇体积分数为70%,提取时间为30min,提取温度为80℃,猪屎豆乙醇提取物对·OH的清除效果最佳。清除率测定体系中,H2O2浓度为0.60%和Luminol浓度为1.00×10-4mol/L时测定结果较准确。结论]猪屎豆乙醇提取物对·OH有很好的清除作用。
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| 关 键 词： | 猪屎豆 抗氧化 鲁米诺 |
Extraction of Antioxidant Active Components of Crotalaria mucronata Desv.and Optimization of Activity Determination System |
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| Institution: | JIANG Pei-pei et al ( Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou, Hainan 571158 ) |
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| Abstract: | Objective]The aim is to study the antioxidant activity of ethanol extracts from Crotalaria mucronata Desv..Method]The ultrasonic-assisted ethanol extraction method is adopted to extract the antioxidant active components of crotalaria mucronata Desv,chemilumines-cence analysis in H2O2-Luminol system is used to determine efficiency of scavenging hydroxyl free radical and optimize the measurement system.Result]Under the conditions that solid-to-liquid ratio is 3.6∶1,ethanol concentration is 70% ,extraction time is 30 min and temperature is 80 ℃,the test results are more accurate and crotalaria mucronata Desv.Shows the best scavenging effects on hydroxyl free radical.Conclusion]The ethanol extract of Crotalaria mucronata Desv..Shows good cavenging effects of hydroxyl free radical. |
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| Keywords: | Crotalaria mucronata Desv Antioxidation Luminol |
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