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| 上海地区宠物犬中戊型肝炎病毒流行病学调查 | |
| 引用本文： | 刘俊峰,华修国,张文,李培锋,杨志彪,沈权.上海地区宠物犬中戊型肝炎病毒流行病学调查[J].安徽农业科学,2008,36(14):5895-5897. |
| 作者姓名： | 刘俊峰 华修国 张文 李培锋 杨志彪 沈权 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018;上海交通大学农业与生物学院,上海市兽医生物技术重点实验室,上海200240 2. 上海交通大学农业与生物学院,上海市兽医生物技术重点实验室,上海200240 3. 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018 |
| 基金项目： | 上海科技攻关计划重点项目 |
| 摘 要： | 目的]为探讨宠物犬在戊型肝炎病毒(HEV)流行环节中的作用提供依据。方法]以散养宠物犬为研究对象,从上海地区及其周边地区随机采集70份血清样品,通过双抗原夹心ELISA试验检测抗HEV抗体,分析不同品种和不同性别宠物犬中血清抗HEV抗体阳性率。利用反转录巢式PCR检测血清中病毒核酸。结果]在70份宠物犬血清样品中,15份样品中抗HEV抗体呈阳性,阳性率为21.4%。上海地区宠物犬阳性率为27.5%,高于上海周边地区。在供试的宠物犬品种中,杜宾犬的HEV抗体阳性率较高(33.3%),其他品种宠物犬为11.1%～25.0%。雌性宠物犬中HEV阳性率为34.4%,显著高于雄性宠物犬。在各种宠物犬中未检测到HEV核酸颗粒。结论]宠物犬对HEV敏感,在HE流行环节中具有不容忽视的作用。 |
| 关 键 词： | 戊型肝炎病毒 反转录巢式PCR 双抗原夹心酶联免疫试验 |
Epidemiological Investigation on the Hepatitis E Virus in Pet Dogs in Shanghai Area |
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| LIU Jun-feng et al.Epidemiological Investigation on the Hepatitis E Virus in Pet Dogs in Shanghai Area[J].Journal of Anhui Agricultural Sciences,2008,36(14):5895-5897. | |
| Authors: | LIU Jun-feng |
| Abstract: | Objective] The research aimed to provide basis for discussing the role of pet dog in the epidemic links of hepatitis E virus.Method] With scatter-fed pet dog as research objects,70 shares of pet dog serum samples were randomly collected from Shanghai area and its surrounding areas.Anti-HEV antibodies were detected in double-antigen sandwich ELISA test to analyze the positive rate of serum anti-HEV antibodies in different varieties and different sexes of pet dogs.The viral RNA in the serum was detected by using RT-nested PCR.Result] Among 70 shares of serum samples of pet dogs,anti-HEV antibodies in 15 samples showed positive,with the positive rate of 21.4%.The positive rate of pet dogs in Shanghai area was 27.5%,higher than that in the surrounding areas of Shanghai.Among the tested varieties of pet dogs,the HEV positive rate of Dubin dog was higher(33.3%),while that of other varieties of pet dogs were 11.1%-25.0%.The HEV infection rate of female pet dogs was 34.4%,significantly higher than that of male pet dogs.HEV RNA granule was not detected in all varieties of pet dog.Conclusion] Pet dog was sensitive to HEV and it had an unnegligible role in the epidemic links of hepatitis E |
| Keywords: | Hepatitis E virus RT-nested PCR Double-antigen sandwich ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |