| 3种鱼类的单个胚胎染色体标本制备方法 |
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| 引用本文： | 王玉生,李雅娟,李佳奇,徐雯,高养春,刘博.3种鱼类的单个胚胎染色体标本制备方法[J].安徽农业科学,2013(23):9832-9832,9840. |
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| 作者姓名： | 王玉生 李雅娟 李佳奇 徐雯 高养春 刘博 |
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| 作者单位： | 大连海洋大学,农业部北方海水增养殖重点实验室辽宁大连116023 |
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| 基金项目： | 国家自然科学基金（31272650）;辽宁省科学技术基金（201102019）. |
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| 摘 要： | 目的]为建立1种准确、快捷、高效的鱼类染色体制备方法.方法]以泥鳅、红鳍东方鲀和德国镜鲤的单个胚胎作为材料,通过秋水仙素、低渗、卡诺固定等操作制备染色体标本.结果]3种鱼类均获得大量形态清晰、长度适中、分散良好的中期分裂相.结论]以鱼类肌肉效应期为材料可成功制备染色体标本,为鱼类染色体标本制备提供了新方法.
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| 关 键 词： | 泥鳅 红鳍东方鲀 德国镜鲤 单个胚胎 染色体 |
Specimen Preparation Methods of Single Embryo Chromosome of Three Kinds of Fish |
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| Institution: | WANG Yu-sheng et al ( Key laboratory of Mariculture ＆ Stock Enhancement in North China Sea, Ministry of Agriculture, Dalian Ocean U- niversity, Dalian, Liaoning 116023) |
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| Abstract: | Objective] The aim was to establish an accurate, raid and high efficient method of preparing fish chromosome. Method] Single embryo of Misgurnus attguillicaudatus, Takifugu rubripes and Cyprinus carpio L. was treated successively with colchicine, hypotonic solution and Carnoy fixation to obtain chromosome specimen. Result] A considerable amount of chromosome of with clear shape, moderate length and good dispersion were obtained from three kinds of fish. Conclusion] Chromosome could be successfully obtained from the embryo sample of the mus- cle effected period, which had given a new way of chromosome preparation. |
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| Keywords: | Misgurnus anguillicaudatus Takifugu rubripes Cyprinus carpio L Single embryo Chromosome |
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