| 红芪多糖的提取分离和含量测定 |
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| 引用本文： | 杨宏梅,李世刚.红芪多糖的提取分离和含量测定[J].安徽农业科学,2013(25):10278-10278,10332. |
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| 作者姓名： | 杨宏梅 李世刚 |
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| 作者单位： | 三峡大学第一临床医学院宜昌市中心医院,湖北宜昌,443002;三峡大学医学院,湖北宜昌,443002 |
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| 基金项目： | 湖北省教育厅项目（B20101201）;宜昌市科技局项目（2010-A01301-04）;三峡大学科研基金项目（0620080702）. |
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| 摘 要： | 目的]对红芪中的多糖进行提取分离和含量测定.方法]采用水提醇沉法提取红芪多糖,并采用苯酚-硫酸法进行含量测定.结果]线性回归方程为：y=0.007X-0.013,R=0.999 6,表明在0～30 mg/L范围内,葡萄糖浓度与吸光度的线性关系良好.红芪药材和红芪精制多糖HPS中的多糖含量分别为4.94％（RSD=1.06％）和45.30％（RSD=1.40％）,平均回收率为99.12％（RSD=0.65％）.结论]该方法简便、快速、准确、灵敏度好,可用于红芪多糖的提取分离和含量测定.
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| 关 键 词： | 红芪多糖 提取分离 含量测定 |
Extraction and Content Determination of Polysaccharides in Hedysayum polybotrys |
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| Institution: | YANG Hong-mei(The First Clinical Medical College of China Three Gorges University, Yichang Central Hospital, Yichang, Hubei 443002) |
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| Abstract: | Objective] To establish a method for extraction and content determination of polysaccharide in Hedysayum polybotrys.Method]Polysaccharide was extracted by water extraction and alcohol precipitation method.The content determination was performed with phenol-sulfuric acid as a chromomeric agent.Result] The linear regression equation was Y =0.007X-0.013,R =0.999 6,indicating within the range of 0-30 mg/L,the linear relationship between glucose concentration and absorbance was good.The content of polysaccharides in Hedysayum polybotrys,and HPS were respectively 4.94％ （RSD =1.06％）,45.308％ （RSD =1.40％）,and the average recovery was 99.12％ （RSD =0.65％）.Conclusion] The method is simple,rapid,accurate,which could be used in extraction and content determination of Hedysayum polybotrys polysaccharide. |
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| Keywords: | Hedysayum polybotrys polysaccharide Extraction and separation Content determination |
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