| 家蚕蛹虫草子实体及大米培养基多糖的提取和纯化 |
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| 引用本文： | 江明珠,颜辉,闻燕,李向梅,祁欣.家蚕蛹虫草子实体及大米培养基多糖的提取和纯化[J].安徽农业科学,2010,38(18):9538-9539,9563. |
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| 作者姓名： | 江明珠 颜辉 闻燕 李向梅 祁欣 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018;江苏科技大学生物与环境工程学院,江苏镇江,212018 |
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| 摘 要： | 目的]为家蚕蛹虫草大米培养基的应用和提高养蚕业的综合经济效益提供基础数据。方法]分别对家蚕蛹虫草子实体及其大米培养基残基进行脱脂、热水浸提、脱蛋白、醇沉、干燥后得到粗多糖,用同种方法提取、纯化,并比较提取、纯化结果。结果]家蚕蛹虫草子实体提取粗多糖得率为25.75%,培养基为12.85%。家蚕蛹虫草子实体和培养基粗多糖经DEAE-纤维素柱纯化,得到相似单一峰;家蚕蛹虫草子实体多糖的洗脱峰部分多糖含量占上样多糖总量的82%;培养基多糖的洗脱峰部分多糖含量占上样多糖总量的74%。两者所含多糖类似,大米培养基粗多糖得率较低,可不提取,有直接作为添加剂等生产应用价值。结论]家蚕蛹虫草大米培养基可以作为一种新型添加剂加入饲料或调味品酿制原料中。
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| 关 键 词： | 家蚕蛹虫草子实体 大米培养基 多糖 提取 纯化 |
Extraction and Purification of the Polysaccharides of Silkworm Cordyceps militaris Fruiting Body and Rice Culture Medium |
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| JIANG Ming-zhu et al.Extraction and Purification of the Polysaccharides of Silkworm Cordyceps militaris Fruiting Body and Rice Culture Medium[J].Journal of Anhui Agricultural Sciences,2010,38(18):9538-9539,9563. |
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| Authors: | JIANG Ming-zhu |
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| Institution: | JIANG Ming-zhu et al(College of Biotechnology and Environment Engineering,University of Science and Technology of Jiangsu,Zhenjiang,Jiangsu 212018) |
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| Abstract: | Objective] The basic data of the comprehensive economic benefits for the application of the rice culture medium for silkworm Cordyceps militaris and the enhancement of silkworm-raising industry was provided through the experiment.Method] The crude polysaccharide from the silkworm Cordyceps militaris sporophore and the rice culture medium,after which was defatted,extracted in hot water,removed in protein,precipitated in alcohol and dried,was obtained.The efficiency of the extraction and purification of the... |
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| Keywords: | Silkworm Cordyceps militaris sporophore Rice medium Polysaccharides Extraction Purification |
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