| 野生与杂交天麻的DNA标记鉴定 |
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| 引用本文： | 王晓丽,陈祖云,王晓玲,宋聚先.野生与杂交天麻的DNA标记鉴定[J].安徽农业科学,2010,38(27):14860-14860,14916. |
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| 作者姓名： | 王晓丽 陈祖云 王晓玲 宋聚先 |
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| 作者单位： | 贵阳医学院药用植物与生药学教研室,贵州贵阳,550004;贵州大学农学院,贵州贵阳,550025 |
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| 基金项目： | 科技部2004BA721A33，黔科合农社字(2001)1125号，黔科合中药专字[2003]51号 |
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| 摘 要： | 目的]从分子水平鉴定野生和杂交天麻。方法]合成SSR特异引物用于天麻基因组DNA的PCR扩增。结果]SSR扩增重复性好、多态性高、分辨力强,野生和杂交天麻有各自特异的DNA标记。其中,杂交天麻均扩增出2条带,为杂合子;野生天麻只扩增出1条带,为纯合子。结论]该研究为野生和杂交天麻种质资源的鉴别、保护、育种提供了一条有效的途径。
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| 关 键 词： | 种质 天麻 SSR DNA标记 |
Identification of Wild and Cultivated Gastrodia elata Bl. by SSR Molecular Marker |
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| Institution: | WANG Xiao-li et al(Department of Medical Plant and Pharmacognosy,Medical College Guiyang,Guiyang,Guizhou 550004) |
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| Abstract: | Objective] Wild and cultivated Gastrodia elata Bl.were identified by SSR molecular marker.Method]Distinctive primers were synthesized to the PCR amplification of the genomic DNA.Result]The number of bands amplified was two in generative propagated individuals which was heterozygote and one in wild and clone propagated individuals which were homozygote.Conclusion]SSR could effectively distinguish heterozygote and homozygote,which would provide effective pathway for the identification and protection of Gastrodia elata Bl.germplasm-resources and select breeding. |
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| Keywords: | SSR |
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