| 马氏珠母贝msi60基因表达量与经济性状的相关分析 |
| |
| 引用本文： | 战欣,于呈呈,石耀华,顾志峰,王爱民.马氏珠母贝msi60基因表达量与经济性状的相关分析[J].安徽农业科学,2016,44(23):84-86. |
| |
| 作者姓名： | 战欣 于呈呈 石耀华 顾志峰 王爱民 |
| |
| 作者单位： | 海南大学海洋学院，热带生物资源教育部重点实验室，海南省热带水生生物技术重点实验室，海南海口570228;海南大学海洋学院，热带生物资源教育部重点实验室，海南省热带水生生物技术重点实验室，海南海口570228;海南大学海洋学院，热带生物资源教育部重点实验室，海南省热带水生生物技术重点实验室，海南海口570228;海南大学海洋学院，热带生物资源教育部重点实验室，海南省热带水生生物技术重点实验室，海南海口570228;海南大学海洋学院，热带生物资源教育部重点实验室，海南省热带水生生物技术重点实验室，海南海口570228 |
| |
| 基金项目： | 国家重点基础研究发展计划(973计划)(2010CB126405)；海南大学科研启动基金项目( kyqd1216)；海南大学青年基金项目(qnjj1227)；海南省青年基金项目(313033)。 |
| |
| 摘 要： | 目的]探讨矿化基因、贝壳和珍珠经济性状之间的相关关系。方法]选择47个培育优质珍珠的马氏珠母贝,测量贝壳的壳宽、壳高、壳长、绞合线长、总壳重、左壳重、珠层厚度及珍珠的直径、重量、珠层厚度,并分析矿化基因msi60在外套膜内缘组织的相对表达量,珍珠经济性状和贝壳经济性状之间的相关关系。结果]珍珠的珠层厚度、珍珠直径和珍珠重量3个经济性状均与受体贝贝壳的壳宽、壳长、总壳重和左壳重呈显著相关,推测育珠贝的生长状况在一定程度上影响珍珠的培育;msi60在外套膜内缘的相对表达量与珍珠重量之间存在相关性(P=0.059),贝壳和珍珠的珍珠层厚度存在显著相关性(P=0.019),推测在马氏珠母贝中,贝壳的矿化和珍珠的矿化可能具有共同的上游调控元件。结论]msi60在形成贝壳珍珠质层的外套膜内缘组织中的基因表达量与珍珠的经济性状相关。该研究为马氏珠母贝生物矿化分子机制的研究提供基础资料。
|
| 关 键 词： | 贝壳 珍珠 经济性状 壳基质蛋白 马氏珠母贝 |
Relationship between Gene Expression of msi60 and Economic Traits in Pinctada fucata martensii |
| |
| Abstract: | Objective] The aim was to analyze the correlations among gene expression levels of msi60,economic trait of shell and economic trait of pearl oyster.Method] A total of 47 pearl oysters that cultured high quality pearls were selected and ten traits (shell width,shell height,shell length,hinge length,total shell weight,left shell weight,thickness of shell nacreous layer,pearl diameter,pearl weight and thickness of pearl na-creous layer) of the selected samples were measured.The correlations among gene expression levels of msi60,economic traits of shell and econom-ic traits of pearl were analyzed.Result] All of the economic traits of pearls were significantly correlated with shell width ,shell length,total shell weight and left shell weight.It was assumed that the growth status of host oyster affects pearl culture.It was found that gene expression levels of msi60 in mantle center were correlated with pearl weight(P=0.059).What’s more,thickness of shell and pearl nacreous layer were significantly correlated with each other(P=0.019).Conclusion] Gene expression of msi60 is correlated with economic traits of pearl.The study will enrich the information for elucidating the molecular mechanism of biomineralization . |
| |
| Keywords: | Shell Pearl Economic trait Shell matrix protein Pinctada fucata martensii |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
