| 三七茎基总皂苷的回流提取工艺研究 |
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| 引用本文： | 赵燕飞,秦枫,江科.三七茎基总皂苷的回流提取工艺研究[J].安徽农业科学,2012(12):7075-7076. |
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| 作者姓名： | 赵燕飞 秦枫 江科 |
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| 作者单位： | 中央广播电视大学农林医药学院,北京,100031;江苏省兽用生物制药高技术研究重点实验室,江苏泰州,225300;青岛市畜牧兽医研究所,山东青岛,266100 |
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| 基金项目： | 江苏省兽用生物制药高技术研究重点实验室开放基金资助(kF 0906) |
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| 摘 要： | 目的]确定三七茎基总皂苷含量测定方法,优化其回流提取工艺。方法]采用香草醛-高氯酸法测定三七茎基总皂苷含量的方法,并以总皂苷含量为指标,优选回流法提取三七茎基总皂苷的工艺条件。结果]在0.008 3～0.049 6 mg/ml浓度范围内,人参皂苷Rg1浓度与吸光度的线性关系良好(R=0.999 4),平均回收率为99.3%(n=4),RSD为3.4%。最佳提取工艺条件为:料液比1∶10(g/ml)、乙醇浓度60%,浸泡时间12 h,提取3次,每次2 h。结论]该含量测定方法简便、准确、重现性好;提取工艺经济、合理,值得进一步推广。
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| 关 键 词： | 三七茎基总皂苷 人参皂苷Rg 香草醛-高氯酸 回流提取 |
Study on the Backflow Extraction Technology of Total Saponins from Stem Base of Panax notoginseng(Burk) F.H. |
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| Institution: | ZHAO Yan-fei et al(Agriculture and Medicine College,China Central Radio and TV University,Beijing 100031) |
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| Abstract: | Objective] To optimize the methods for content determination and backflow extraction technology of total saponins in stem base of Panax notoginseng(Burk) F.H..Method] The content of total saponins was determined by the methods of vanillin-perchloric acid and the extraction technology was optimized by the content of total saponins.Result] Panax saponin Rg1 presented good linear relationship over the range of 0.008 3 to 0.049 6 mg/ml(r=0.999 4).The average recovery was 98.6% with the RSD of 3.54%(n=4) respectively.The optimal solvent backflow extraction conditions were as follows:P.notoginseng stem base was soaked for 12 h in 60% ethanol at the solid-liquid ratio of 1∶ 10 g/ml and extracted for 3 times for 2 h each time.Conclusion] This method is simple and feasible,highly accurate and precise.The extraction process is economical,reasonable and deserved popularizing all the more. |
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| Keywords: | Total saponins in P notoginseng stem base Panax saponin Rg1 Vanillin-perchloric acid Backflow extraction |
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