| 胭脂鱼精子低温保存及其在人工授精中的应用 |
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| 引用本文： | 万全,李飞.胭脂鱼精子低温保存及其在人工授精中的应用[J].安徽农业科学,2009,37(3):1122-1123. |
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| 作者姓名： | 万全 李飞 |
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| 作者单位： | 安徽农业大学动物科技学院,安徽合肥,230036 |
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| 基金项目： | 三峡工程珍稀鱼类保护生态补偿增殖放流项目 |
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| 摘 要： | 目的]研究胭脂鱼精子的低温保存方法，为胭脂鱼的人工繁殖和人工授精提供基础依据。方法]试验配制了A、B、C、D4种精子保存液，以0．7％的NaCl溶液作为对照，以精子被激活后的活率为指标，对4种精子保存液保存成熟的胭脂鱼精子的效果进行评价。结果]在低温（0～4℃）条件下，4种精子保存液之间及其与对照溶液之间的差异比较明显。其中保存效果最好的是B液，能在120h内使精子的活率保持为80％；D液次之，能在84h内使精子的活率保持为80％。胭脂鱼在淡水中快速运动时间为（24．0±3．5）s，寿命为（128．0±9．5）s。2007～2008年精子保存B液在胭脂鱼的人工授精中进行应用，受精率为53％～85％。结论]用精子保存液低温保存胭脂鱼精子的方法是切实可行的。
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| 关 键 词： | 胭脂鱼 精子 低温保存 人工授精 |
Study on the Application of Spermatozoa Preservation at Low Temperature in Artificial Fecundation of Myxocyprinus asiaticus Spermatozoa |
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| Institution: | WAN Quan et al (College of Animal Science and Technology ,Anhui Agricultural University,Hefei,Anhui 230036) |
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| Abstract: | Objective] The study aimed to investigate the method of low temperature preservation for Myxocyprinus asiaticus spermatozoa and provide the foundation data for artificial reproduction and fecundation.Method] In the test,4 kinds of spermatozoa preservative fluids of A,B,C and D were prepared,with 0.7% NaCl solution as CK.The effects of four preservative fluids of mature spermatozoa from M.asiaticus were evaluated with the fast moving rate of spermatozoa after being motivated as an index.Result] Under the low temperature of 0-4 ℃,the differences between 4 kinds of the preservative fluids and between them and CK fluids were more obvious.Among them,B fluid was the best,which could keep 80% of spermatozoa having fast moving ability within 120 h and D fluid was second,which could keep 80% of spermatozoa having fast moving ability within 84 h.The moving time of M.asiaticus spermatozoa in fresh water was(24.0±3.5) s,with the life time of(128.0±9.5) s.From 2007 to 2008,when the permatozoa preservative B fluid was used in the artificial insemination of M.asiaticus,the fecundation was 53%-85%.Conclusion] The method of keeping M.asiaticus spermatozoa at low temperature with spermatozoa preservative fluids was feasible. |
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| Keywords: | Myxocyprinus asiaticus Spermatozoa Low temperature preservation Artificial fecundation |
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