| 奶牛子宫内膜炎病原菌的分离·鉴定及药敏试验 |
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| 引用本文： | 马建玲.奶牛子宫内膜炎病原菌的分离·鉴定及药敏试验[J].安徽农业科学,2014(14):4371-4373. |
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| 作者姓名： | 马建玲 |
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| 作者单位： | 新疆库尔勒市动物防疫监督站; |
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| 摘 要： | 目的]了解奶牛子宫内膜炎的病原菌感染情况与耐药现状.方法]随机采集库尔勒地区某牛场患子宫内膜炎奶牛的子宫黏液20份,进行实验室细菌学的分离、生化鉴定及药敏试验.结果]共分离出89株菌,其中链球菌45株,占50.56％;葡萄球菌24株,占26.97％;大肠杆菌13株,占14.60％;蜡样芽胞杆菌7株,占7.87％.分离出的单一菌株的检出率为80％ （16/20）.引起该地区奶牛子宫内膜炎的主要病原菌是链球菌、葡萄球菌.对链球菌、葡萄球菌、大肠杆菌、蜡样芽胞杆菌这4种菌抑菌效果最好的药物依次分别为奥复星、氟苯尼考、甲氧苄啶、氟苯尼考.结论]该研究可为指导子宫内膜炎的临床用药和预防提供理论依据与技术支持.
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| 关 键 词： | 子宫内膜炎 分离 鉴定 药敏试验 |
Separation,Identification and Drug Sensitivity Test of Pathogenic Bacteria of Endometritis of Dairy Cattle |
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| MA Jian-ling.Separation,Identification and Drug Sensitivity Test of Pathogenic Bacteria of Endometritis of Dairy Cattle[J].Journal of Anhui Agricultural Sciences,2014(14):4371-4373. |
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| Authors: | MA Jian-ling |
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| Institution: | MA Jian-ling(Korla Animal Quarantine Surveillance Station in Xinjiang, Korla, Xinjiang 841000) |
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| Abstract: | Objective] The research aimed to infection situations of pathogenic bacteria of endometritis of dairy cattle and their present drug tolerance.Method] 20 samples of uterus mucus of dairy cattle with endometritis in a cattle farm in Korla were randomly collected to make the bacterial separation and identification in laboratory and drug-sensitive test.Result] 89 bacterial strains were isolated,including 45 strains of Streptococcus sp.（50.56％）,24 strains of Staphylococcus aureus （26.97％）,13 strains of Escherichia coli（14.60％）,7 strains of Bacillus cereus （7.87％）.The detection rate of single strain separated was 80％ （16/20）.The main pathogenic bacteria that caused endometritis of dairy cattle were Streptococcus sp.and S.aureus.The drugs with the best antibacterial effect on Streptococcus sp.,S.aureus,E.coli and B.cereus were ofloxacin,florfenicol,trimethoprim and florfenicol.Conclusion] The research could provide theoretical basis and technical support for guiding the drug administration in the clinic and prevention of endometritis. |
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| Keywords: | Endometritis Isolation Identification Drug-sensitive test |
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