| PCR－核酸试纸条法快速检测鸽子源性成分 |
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| 引用本文： | 田卓,曹际娟,崔妍,赵良娟,李宗梦.PCR－核酸试纸条法快速检测鸽子源性成分[J].安徽农业科学,2016,44(20). |
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| 作者姓名： | 田卓 曹际娟 崔妍 赵良娟 李宗梦 |
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| 作者单位： | 大连工业大学食品学院，辽宁大连116034;大连出入境检验检疫局，辽宁大连116400;大连工业大学食品学院，辽宁大连116034;辽宁出入境检验检疫局，辽宁大连116001;大连出入境检验检疫局,辽宁大连,116400;天津出入境检验检疫局,天津,300201 |
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| 基金项目： | 国家质检总局课题项目(2014IK105)。 |
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| 摘 要： | 目的]提高动物源性成分检测效率,建立PCR-核酸试纸条快速检测技术体系。方法]针对物种特异性DNA序列,设计并标记引物;建立并优化PCR反应体系;利用通用核酸试纸条进行结果判读。结果]试验表明,PCR-核酸试纸条特异性高;绝对灵敏度为0.001 3 ng/μL,混合样品,实际灵敏度为0.01%;加工处理样品,实际灵敏度为0.1%;最低检测限检出率为100%,稳定性良好。结论]综上,PCR-核酸试纸条方法是一种既有PCR反应的高灵敏度,又具有免疫学检测的特异性好的特点,同时操作简便的快速检测方法。
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| 关 键 词： | 核酸试纸条 动物源 食品安全 |
Rapid Detection of Dove Origin Ingredients by PCR-Nucleic Acid Test Paper |
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| Abstract: | Objective] In order to improve the efficiency of detecting ingredients of animal origin, the PCR-nucleic acid strip rapid detection technology system was established. Method] In view of the species specific DNA sequences, primers were designed and marked;the PCR re-action system was established and optimized; the results were interpretated by the nucleic acid strip. Result] The results showed that: the PCR-nucleic acid strip is high specificity;absolute sensitivity is 0.001 3 ng/μL;the actual sensitivity in mixed samples is 0.01%;the actual sensitivity in processing sample is 0.1%;the detection rate of minimum detection limit is 100%, the stability is good.Conclusion] To sum up, the high sensitivity as PCR and high specfticity as immunology made the PCR-chromatography strip valuable for the rapid detection method. |
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| Keywords: | Nucleic acid test paper Animal origin Food safety |
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