| 菝葜组织培养研究 |
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| 引用本文： | 郭永庆,王奎玲,刘庆华,刘庆超.菝葜组织培养研究[J].安徽农业科学,2008,36(13):5295-5296. |
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| 作者姓名： | 郭永庆 王奎玲 刘庆华 刘庆超 |
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| 作者单位： | 青岛农业大学环境艺术学院,山东青岛,266109 |
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| 基金项目： | 山东省农业良种工程重大项目 |
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| 摘 要： | 目的]更好地开发利用菝葜。方法]以菝葜幼嫩茎段为外植体,以MS1、/2 MS1、/4 MS为基本培养基,研究不同植物激素组合对菝葜组织培养快速繁殖的影响。结果]外植体的最佳消毒方式为0.1%升汞消毒9 min。1/2 MS1、/4 MS培养基更有利于菝葜芽的诱导、增殖及生根,6-BA对芽丛的增殖培养具有关键作用。最佳启动培养基为1/2 MS+6-BA 2.0 mg/L+IBA 0.1 mg/L+NAA 0.2 mg/L。最佳增殖培养基为1/2 MS+6-BA 3.0 mg/L+IBA 0.2 mg/L+NAA 0.2 mg/L。最佳生根培养基为1/4 MS+IBA 0.1 mg/L+NAA 1.5 mg/L+活性炭1 000 mg/L,生根率最高,达75%。结论]该研究建立了菝葜的组织培养繁殖体系,增加了菝葜的繁殖系数。
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| 关 键 词： | 菝葜 组织培养 植物激素 外植体 |
Study on the Technology of Tissue Culture on Smilax china L. |
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| GUO Yong-qing et al.Study on the Technology of Tissue Culture on Smilax china L.[J].Journal of Anhui Agricultural Sciences,2008,36(13):5295-5296. |
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| Authors: | GUO Yong-qing |
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| Abstract: | Objective] The aim of the research was to develop and utilize Smilax china L.better.Method] With tender stems of S.china as explants,MS,1/2 MS and 1/4 MS were taken as basic medium to study the effects of different plant hormone combinations on the tissue culture and rapid propagation of S.china.Result] The optimum disinfection method of explants was disinfecting with 0.1% mercuric chloride for 9 min.Medium 1/2 MS and 1/4 MS were more favorable for the bud induction,propagation and rooting of S. china.6-BA played a key effect on the propagation culture of bud clumping.The optimum initial medium was 1/2 MS 6-BA 2.0 mg/L IBA 0.1 mg/L NAA 0.2 mg/L.The optimum propagation medium was 1/2 MS 6-BA 3.0 mg/L IBA 0.2 mg/L NAA 0.2 mg/L.The optimum rooting medium was 1/4 MS IBA 0.1 mg/L NAA 1.5 mg/L activated carbon 1 000 mg/L,with the rooting rate being highest(75%).Conclusion] The tissue culture and propagation system was established and the propagation coefficient of S.china was increased in this research. |
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| Keywords: | Smilax china L Tissue culture Plant hormone Explant |
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