| 运用致病毒素筛选抗稻曲病突变体的研究 |
| |
| 引用本文： | 马慧,张丽,钟鸣,郭志富,王学英,陈丽静.运用致病毒素筛选抗稻曲病突变体的研究[J].安徽农业科学,2007,35(16):4746-4747. |
| |
| 作者姓名： | 马慧 张丽 钟鸣 郭志富 王学英 陈丽静 |
| |
| 作者单位： | 沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161;沈阳农业大学生物科学技术学院,辽宁省农业生物技术重点实验室,辽宁沈阳,110161 |
| |
| 摘 要： | 通过4个品种水稻愈伤组织的诱导、继代与分化,研究了稻曲病病原菌粗毒素为选择压力进行抗稻曲病突变体的筛选的可行性。结果表明,供试的水稻各品种在细胞水平的抗性与田间的抗性相一致,因而可以以制备的稻曲病菌粗毒素为选择压力进行抗病突变体的筛选。对得到的抗性愈伤组织的抗性稳定性进行了鉴定。结果表明,继代后愈伤组织的抗性仍得到了保持并得到了分化植株。这说明毒素筛选体系是目前最成熟的一种方法。
|
| 关 键 词： | 水稻 抗性筛选 稻曲病 突变体 |
| 文章编号： | 0517-6611（2007）16-04746-02 |
| 修稿时间： | 2007-03-05 |
Screening Mutant against False Smut Disease by Using Pathotoxin |
| |
| MA Hai et al.Screening Mutant against False Smut Disease by Using Pathotoxin[J].Journal of Anhui Agricultural Sciences,2007,35(16):4746-4747. |
| |
| Authors: | MA Hai |
| |
| Institution: | College of Biology Science and Technology, Shenyang Agricultural University, Key Laboratory of Agricuhural Biotechnology of Liaoning Province, Shenyang, Liaoning 110161 |
| |
| Abstract: | The induction,subculture and differentiation of the callus of four paddy rice varieties were conducted to study the feasibility of screening the mutant against false smut disease with the crude toxin of Ustilaginoidea virens(Cke.) Tak.as the selection pressure.The result indicated that the resistance of cell level was consistent with resistance in the field among the tested rice varieties.Thus,the prepared crude toxin of Ustilaginoidea virens could be used as selection pressure to screen the mutant with disease resistance.The identification on the resistance stability of the obtained callus with resistance showed that the resistance of callus after subculture could still be maintained and the differentiation plantlets were obtained,suggesting that the toxin screening system was the most mature method at present. |
| |
| Keywords: | Rice Resistant screening False smut disease Mutant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
