| 圆鼻巨蜥遗传多样性的RAPD分析 |
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| 引用本文： | 傅美兰,于冬梅,王莹,彭建军.圆鼻巨蜥遗传多样性的RAPD分析[J].安徽农业科学,2011(13):7842-7845. |
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| 作者姓名： | 傅美兰 于冬梅 王莹 彭建军 |
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| 作者单位： | 暨南大学生命科学技术学院;华南濒危动物研究所物种鉴定中心; |
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| 基金项目： | 广东省自然科学基金项目(9151026001000003); 广东省科学院优秀青年科技人才基金项目(200704); 广东省野生动物保护和利用公共实验室基金项目(200901) |
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| 摘 要： | 目的]采用RAPD技术对圆鼻巨蜥（Varanus salvator）进行遗传多样性分析。方法]用20个随机引物对圆鼻巨蜥36个个体的基因组DNA进行PCR扩增。结果]有10对引物能扩出清晰稳定的条带,共扩增出2952条DNA片段,平均每个个体扩增出82个条带,其中47条具多态性,多态性位点比为57.32%,36个个体间遗传距离为0.035 9~0.335 9,平均遗传距离为0.135 92。Nei s基因多样性指数H=0.181 9,Shannon s多样性指数I=0.263 0,表明圆鼻巨蜥具有较高的遗传多样性。结论]采用UPGMA法构建了36个个体相互关系的分子聚类图,发现没有形成明显的种群分化。
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| 关 键 词： | 圆鼻巨蜥 遗传多样性 RAPD |
RAPD Analysis for Genetic Diversity of Varanus salvator |
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| FU Mei-lan et al.RAPD Analysis for Genetic Diversity of Varanus salvator[J].Journal of Anhui Agricultural Sciences,2011(13):7842-7845. |
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| Authors: | FU Mei-lan |
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| Institution: | FU Mei-lan et al(Life Science and Technology Institute,Ji'nan University,Guangzhou,Guangdong 510632) |
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| Abstract: | Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator.Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V.salvator.Result] 10 primers could produce highly reproducible RAPD bands.A total of 2 952 DNA fragments were successfully amplified.Each individual got 82 amplified bands on average,47 of which showed polymorphism.The polymorphic locus percent was 57.32%.The genetic distance among 36 individuals ranged from 0.035 9 to 0.... |
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| Keywords: | Varanus salvator Genetic diversity RAPD UPGMA analysis |
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