| 绵羊体表脓包病原的分离·鉴定及药敏试验 |
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| 引用本文： | 张晓,刘志杰,杨吉飞,陈泽,关贵全,刘爱红,任巧云,罗建勋,殷宏,李有全.绵羊体表脓包病原的分离·鉴定及药敏试验[J].安徽农业科学,2014(17):5503-5505. |
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| 作者姓名： | 张晓 刘志杰 杨吉飞 陈泽 关贵全 刘爱红 任巧云 罗建勋 殷宏 李有全 |
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| 作者单位： | 中国农业科学院兰州兽医研究所;家畜疫病病原生物学国家重点实验室/甘肃省寄生虫学重点实验室/农业部草食动物疫病重点实验室; |
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| 基金项目： | 国家自然科学基金项目(31272556,31101621,30972182,31072130,31001061);“973”项目(2010CB530206);甘肃省青年自然基金(1107RJYA067);“948”项目(2010-S04);甘肃省重点项目(1002NKDA035);农业部肉牛牦牛产业体系项目(CARS-38);欧盟-科技部合作专项[欧盟EPIZONE(FOOD-CT-2006-016236),ASFRISK(211691),ARBOZOO-NET(211757),PIROVAC(KBBE-3-245145)] |
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| 摘 要： | 目的]对分离自湖北省大悟县的患病山羊体表脓包中的致病菌进行分析和鉴定，以确定脓包中细菌的分子分类学地位及药物敏感性。方法]通过对分离株16SrRNA基因序列分析，结合细菌菌落形态和细菌生化特性分析进行细菌分离和鉴定，并对已鉴定的菌株进行药物敏感性试验，筛选出脓包细菌敏感性药物。结果]从脓包分离的细菌分别为葡萄球菌属、大肠埃希氏杆菌属、不动杆菌属以及绿脓杆菌；药物敏感试验表明这些细菌对药物的敏感性不同，其中对头孢类、喹诺酮类、氨基糖苷类以及氯霉素等敏感。结论]该研究可为该地区该病流行病学的研究与防治药物的研发提供参考。
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| 关 键 词： | 山羊 脓包 16S rRNA 药物敏感性 鉴定 |
Pathogen Identification and Susceptibility Analysis of Sheep Surface Pustule |
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| Institution: | Zhang Xiao, LI You-quart et al ( Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Labora- tory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansn Province/Key Laboratory of Grazing Animal Disease of Ministry of Agriculture, Lanzhou, Gansu 730046 ) |
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| Abstract: | Objective ] Pathogens isolated from pustule of goat surface in Hubei Dawu were analyzed and identified to determine its molecular taxonomic status and drug sensitivity. Method] Isolates were isolated and identified according to the gene sequence of 16S rRNA, combining with morphological and biochemical characteristics. Then drug susceptibility tests were made to screen sensitive drug strains. Result] Pseud-omonas sp. , Escherichia coli, Acinetobacter sp. , Lysinibacillus sp. were isolated from the pustules in terms of the identification results; And drug susceptibility tests showed that these bacterias are sensitivity to eephalosporins, quinolones, aminoglycosides, and chloramphenicol. Conclusion] The study can provide some references for the drug development and epidemiologie study of this area. |
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| Keywords: | Goat Pustule 16S ribosomal RNA gene Drug susceptibility Identification |
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