| 张家口地区仔猪白痢病原菌血清型检测及药敏试验 |
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| 引用本文： | 顾小龙,刘红彬,张鹤亮,方素芳,崔平.张家口地区仔猪白痢病原菌血清型检测及药敏试验[J].安徽农业科学,2012(30):14766-14768. |
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| 作者姓名： | 顾小龙 刘红彬 张鹤亮 方素芳 崔平 |
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| 作者单位： | 河北北方学院动物科技学院,河北张家口,075000;河北北方学院药学系,河北张家口,075000 |
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| 基金项目： | 河北省教育厅项目(2010216);张家口市科技局项目(1012004C-2) |
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| 摘 要： | 目的]为临床上选用仔猪白痢疫苗与防治用药提供参考。方法]从20个规模化猪场采集40份仔猪白痢病料,对致病性大肠杆菌进行分离和生化鉴定,分析其血清型并检测其耐药性。结果]从40份病料中分离出36株大肠杆菌,其中32株为致病性大肠杆菌。分离菌对16种药物均有不同程度的耐药性,最高为100%,最低为6%。所有分离菌均表现出不同程度的多重耐药性,其中22株分离菌为4耐以上的菌株。32株致病菌分布在9个血清型中。结论]张家口地区规模化猪场仔猪白痢病原菌的优势血清型为O8、O149、O141和O54,占已定型菌株的59.3%。对仔猪白痢病原菌敏感率较高的药物为头孢噻肟钠、诺氟沙星、丁胺卡那和新霉素。
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| 关 键 词： | 仔猪白痢 大肠杆菌 血清型 药敏试验 |
Serotype Detection and Drug Sensitivity Test of White Scour of Piglets Pathogen in Zhangjiakou Area |
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| Institution: | GU Xiao-long et al(College of Animal Science and Technology,Hebei North University,Zhangjiakou,Hebei 075000) |
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| Abstract: | Objective] The research aimed to provide references for selecting bacterin and drugs for white scour of piglet in the clinic.Method] 40 materials of piglets with white scour were collected from 20 large-scale pig farms and pathogenic Escherichia coli were isolated and biochemical identification was conducted.The serotypes of the isolated strains were analyzed and the drug resistance was detected.Result] 36 strains of E.coli were isolated from 40 disease materials.Among them,32 strains were pathogenic E.coli.The isolated strains showed different drug resistance to 16 kinds of drugs,with the drug resistance rate of 6%-100%.All the isolated strains showed different multiple antibiotic resistance and 22 strains showed resistance to more than 4 kinds of drugs.32 pathogenic strains were distributed in 9 kinds of serotypes.Conclusion] The predominant serotypes of white scour pathogen of piglet in large-scale pig farms of Zhangjiakou area were O8,O151,O149 and O141,which accounted for 59.3% of all identified bacteria.The drugs with higher sensitivity to white scour pathogen of piglet were cefotaxime sodium,norfloxacin,amikacin and neomycin. |
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| Keywords: | White scour of piglet E coli Serotype Drug sensitivity test |
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