| 冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育 |
| |
| 引用本文： | 程果,李学军,乔志刚,石灵,彭新亮.冷休克诱导黄河鲇(Silurus asotus)二倍体雌核发育[J].安徽农业科学,2008,36(23). |
| |
| 作者姓名： | 程果 李学军 乔志刚 石灵 彭新亮 |
| |
| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007 |
| |
| 基金项目： | 河南省科技攻关项目，河南省重点学科建设项目 |
| |
| 摘 要： | 目的]通过冷休克法诱导黄河鲇二倍体雌核发育。方法]在水温23.5±0.5℃的条件下,用紫外线照射黄河鲇(Silurus asotus)精子,然后进行进行人工受精,设置不同的冷休克起始时间和持续处理时间,观察黄河鲇二倍体雌核发育。结果]在紫外线照射15min、冷休克起始时间为受精后5 min、持续处理40 min的条件下得到的二倍体黄河鲇成活率最高,达8.5%。结论]该研究为培育优良黄河鲇新品种奠定了基础,为深入研究雌核发育机理积累了原始资料。
|
| 关 键 词： | 黄河鲇 雌核发育 二倍体 冷休克 |
Induction on Gynogenesis of Cold Shock in Silurus astus |
| |
| CHENG Guo et al.Induction on Gynogenesis of Cold Shock in Silurus astus[J].Journal of Anhui Agricultural Sciences,2008,36(23). |
| |
| Authors: | CHENG Guo |
| |
| Abstract: | Objective]The cold shock method was used to induce diploid gynogenesis of Silurus astus. Silurus astus sperms were irradiated by ultraviolet then conducted fertilization.Different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus astus.Result]Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization and cold shock duartion was 40 min,the survival rate of diploid Silurus astus was highest(8.5%)Conclusion]The experiment laid foundation for culturing new excellent Silurus astus varieties and accumulated original files for further study of gynogenesisdevelopment mechanism. |
| |
| Keywords: | Silurus astus Gynogenesis Diploid Cold shock |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
