| 高效液相色谱法检测鳗鱼中孔雀石绿及其代谢物残留 |
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| 引用本文： | 李永夫,高华鹏,姚芳,殷居易.高效液相色谱法检测鳗鱼中孔雀石绿及其代谢物残留[J].安徽农业科学,2009,37(1):31-33. |
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| 作者姓名： | 李永夫 高华鹏 姚芳 殷居易 |
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| 作者单位： | 浙江林学院环境科技学院,浙江临安,311300;徐龙食品集团有限公司,浙江宁波,315300;浙江省宁波出入境检验检疫局,浙江宁波,315012 |
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| 基金项目： | 浙江林学院科学研究发展基金(2351000731) |
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| 摘 要： | 目的]采用高效液相色谱法测定鳗鱼中孔雀石绿及其代谢物隐色孔雀石绿残留量。方法]鳗鱼样品用乙腈提取，经脱脂、浓缩、净化和流动相溶解，用高效液相色谱二极管阵列检测器（DAD）测定。结果]在HPLC条件下，14min内可完成对孔雀石绿和隐色孔雀石绿的测定。在0～0．1μg/ml浓度范围内，孔雀石绿和隐色孔雀石标样工作曲线的相关系数（r）分别为0．9999和0．9998；在2、25、100μg/kg添加水平条件下，孔雀石绿、隐色孔雀石绿的加标回收率分别为85．7％～89．7％和87．6％～91．2％，测定相对标准偏差（RSD）小于5％（n=5）；该方法对孔雀石绿和隐色孔雀石绿的检测限分别为1和2μg／kg（S／N≥3）。结论]该研究应用HPLC测定鳗鱼中孔雀石绿等残留准确、可靠，其灵敏度符合法规要求。
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| 关 键 词： | 高效液相色谱 孔雀石绿 隐色孔雀石绿 鳗鱼 残留检测 |
Detection on the Residues of Malachite Green and Its Metabolite in Eel by High Performance Liquid Chromatography |
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| LI Yong-fu et al.Detection on the Residues of Malachite Green and Its Metabolite in Eel by High Performance Liquid Chromatography[J].Journal of Anhui Agricultural Sciences,2009,37(1):31-33. |
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| Authors: | LI Yong-fu |
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| Institution: | LI Yong-fu et al (College of Environmental Technology,Zhejiang Forestry University,Linan,Zhejiang 311300) |
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| Abstract: | Objective] The study aimed to detect the residues of malachite green(MG) and its metabolite leucomalachite malachite green(LMG) in eel by high performance liquid chromatography(HPLC).Method] The eel sample was extracted by acetonitrile and was finally dissolved in the mobile phase after the procedure of being defatted,concentrated and purified.HPLC with diode array detector(DAD) was used for the determination.Result] The detection on the residues of MG and its metabolite LMG in eel was completed within 1... |
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| Keywords: | High performance liquid chromatography Malachite green Leucomalachite malachite green Eel Residue detection |
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