| 利用PCR技术快速检测水产品中副溶血性弧菌 |
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| 引用本文： | 王豪,孙晓红.利用PCR技术快速检测水产品中副溶血性弧菌[J].安徽农业科学,2009,37(15):6909-6910. |
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| 作者姓名： | 王豪 孙晓红 |
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| 作者单位： | 上海海洋大学食品学院,上海,201306;上海海洋大学食品学院,上海,201306 |
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| 摘 要： | 目的]对副溶血性弧菌的检测进行研究。方法]采用聚合酶链式反应（PCR），以标准菌株（单增李斯特菌、哈氏弧菌）作为阴性对照对3株副溶血性弧菌进行特异性扩增，扩增引物采用副溶血性弧菌如基因中的tlh-3和tlh-4，同时利用PCR对人工污染的白对虾中的副溶血性弧菌进行检出限测定。结果]结果表明，通过PER扩增，副溶血性弧菌在约449bp处扩增出特异性条带，单增李斯特菌、哈氏弧菌均未出现任何条带，扩增片段表现出极好的特异性。菌液稀释到3．3×10^2cfu／ml时，将其污染到白对虾中作PCR仍可扩增出目的片断。结论]该研究表明PER检测方法快捷、特异性好、敏感性高，可以作为副溶血性弧菌辅助检测方法。
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| 关 键 词： | 副溶血性弧菌 PCR 检出限 |
Rapid Detection of Vibrio parahaemolyticus in Aquatic Products by PCR Technology |
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| Institution: | WANG Hao et al (Food College of Shanghai Ocean University,Shanghai 201306) |
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| Abstract: | Objective]The research aimed to study the detection of Vibrio parahaemolyticus.Method]Three V.parahaemolyticus strains were specially amplified by PCR,and the standard referenced strains used for negative comparison were Listeria monocytogenes and V.harveyi.The primers of amplification were tlh-3 and tlh-4 gene in V.parahaemolyticus.At the same time,detection limits of manual contaminated prawns were determined with PCR.Result]The results showed that,V.parahaemolyticus amplified special segment in 449 bp but L.monocytogenes and V.harveyi didn't.The amplified segment performed splendiferous specialty.By PCR amplification,the aimed snippet could be also amplified when bacteria solution was diluted to 3.3×102 cfu/ml and contaminated to prawns.Conclusion]This research proved that PCR method is a rapid,special,and highly sensitive technology. |
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| Keywords: | Vibrio parahaemolyticus PCR Detection limit |
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