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盐处理对碱蓬生长及叶绿体超微结构的影响
引用本文:王文慧,张全星,刘,婧等.盐处理对碱蓬生长及叶绿体超微结构的影响[J].安徽农业科学,2013(23):9531-9533.
作者姓名:王文慧  张全星    婧等
作者单位:德州学院生命科学学院生物技术与生物资源利用山东高校重点实验室,山东德州253023
基金项目:德州市科技计划资助项目(20090162-1);山东省高校科研发展计划项目(J13LE12).
摘    要:目的]研究盐处理对碱蓬生长及叶绿体超微结构的影响.方法]用含有0、100、200、500 mmol/L NaCl的溶液处理碱蓬幼苗,处理10 d后测定幼苗鲜重、干重、叶绿素含量、光合速率、叶绿体超微结构、气孔导度及细胞间隙CO2浓度等指标.结果]一定浓度的NaCl促进了碱蓬的生长.200 mmol/L NaCl是碱蓬生长的最适盐度,使Pn升高,Gs升高,Gi升高,叶绿体结构未受影响;而500 mmol/LNaCl使Pn、Gs降低,Ci升高,叶绿体超微结构受到破坏.盐处理条件下叶绿素a含量先升高后降低,叶绿素b含量先降低后升高.结论]高盐度时碱蓬光合速率的降低是由非气孔因素引起的.

关 键 词:碱蓬  叶绿素  光合速率  叶绿体超微结构

Effect of NaCl Stress on Growth and Ultrastructure of Chloroplast of Suaeda salsa
Institution:WANG Wen-hui et al ( Key Laboratory of Biotechnology and Biological Resource Utilization in Universities of Shandong, College of Life Sci- ence, Dezhou University, Dezhou, Shandong 253023)
Abstract: Objective ] The aim was to study effect of NaC1 stress on growth and ultrastructure of chloroplast of Suaeda salsa. Method ] Seed- iings of Su, aeda salsa were treated with different NaCI(0, 100,200,500 retool/L) for 10 days, then the fresh weight, dry weight, photosynthesis rate, ultrastructure of chloroplast and other parameters were measured. Result ] NaC1 could promote the growth of Suaeda salsa. When NaC1 concentration was about 200 mmol/L, Suaeda salsa grew the strongest. Photosynthesis rate Ci, and Gs increased with the salinity under 100 -200 mmol/L NaCL Photosynthesis rate and Gs declined under 500 mmol/L NaC1, but C~ increased. Uitrastructure of chloroplast was destroyed by 500 NaC1 mmol/L. After salt treatment, Chla contents increased firstly, then increased with the increasing of NaC1 concentrations, but Chlb content declined under 0 - 100 mmol/L NaC1 and increased under 500 mmol/L NaC1. Conclusion] The decrease of photosynthetic rate was caused by non stomatal factors under high salinity stress.
Keywords:Suaeda salsa  Phchlorophyll  Photosynthetic rate  Uhrastructure of chloroplast
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