| T1083替换NtKRP尾部BD融合载体pGBKT7-Ts的构建 |
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| 引用本文： | 纪耀坤,张伟彬,袁亮.T1083替换NtKRP尾部BD融合载体pGBKT7-Ts的构建[J].安徽农业科学,2010,38(13):6658-6659,6685. |
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| 作者姓名： | 纪耀坤 张伟彬 袁亮 |
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| 作者单位： | 商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100;商丘职业技术学院,河南商丘,476100 |
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| 摘 要： | 目的]构建T1083替换NtKRP尾部BD融合载体pGBKT7-Ts。方法]以pBI121KE质粒为模板,通过重叠PCR扩增烟草NtKRP基因尾部含T1083替换的片段,将其克隆入pGBKT7载体。结果]成功构建了T1083替换突变BD融合质粒载体pGBKT7-Ts。结论]为进一步研究NtKRP尾部T1083替换对NtKRP与靶物质结合的影响奠定了实验基础。
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| 关 键 词： | NtKRP pGBKT7-Ts 融合载体 |
Construction of BD Fusion Vector pGBKT7 Ts with T 1083 Substitution in of NtKRP Tail |
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| Institution: | JI Yao-kun et al (Shang Qiu Polytechnic,Shangqiu,Henan 476100) |
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| Abstract: | Objective]The aim was to construct BD fusion vector pGBKT7-Ts with T1083 substitution in NtKRP tail. Method]T1083 substitution mutant in tail sequence of tobacco NtKRP was amplified by overlapping PCR from pBI121KE plasmid template,and the fragment was cloned into pGBKT7 vector. Result]BD fusion expression plasmid pGBKT7-Ts was constructed successfully. Conclusion]The study laid an experimental foundation for further understanding of the effect of T1083 substitution mutation on the interaction of NtKRP and its target partner. |
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| Keywords: | NtKRP pGBKT7-Ts |
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