| 攸县油茶胚状体发生及其组织学观察 |
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| 引用本文： | 彭秋发,戴亚娟,杜跃强,吕洪飞.攸县油茶胚状体发生及其组织学观察[J].安徽农业科学,2008,36(8):3203-3204. |
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| 作者姓名： | 彭秋发 戴亚娟 杜跃强 吕洪飞 |
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| 作者单位： | 浙江师范大学生化学院,浙江,金华,321004;浙江省金华市林业局,浙江,金华,321004 |
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| 摘 要： | 目的]研究以攸县油茶(Camellia yuhsienensis Hu)成熟子叶为外植体的胚状体发生情况。方法]以子叶为外植体,接种于MS+2.0mg/L2,4-D+1.0mg/LKT培养基上,诱导胚性愈伤组织。胚性愈伤组织块转接至以MS为基本培养基,添加不同浓度6-BA和NAA的激素组合的培养基上诱导产生胚状体。取不同发育阶段的组织块制作石蜡切片,进行组织学观察。结果]成熟子叶块接种于诱导培养基MS+2.0mg/L2,4-D+1.0mg/LKT,15d左右开始出现胚性愈伤组织。胚性愈伤组织转接至培养基为MS+2.0mg/L6-BA+0.6mg/LNAA时胚状体诱导率最高,为87.78%;无激素培养基也能诱导胚性愈伤组织产生胚状体,但诱导率极低,仅为6.67%。组织学观察表明,胚性细胞由愈伤组织表面及其内部邻近的若干细胞分化而来,经过球形胚、心形胚、子叶形胚等不同发育阶段直至成熟胚状体。结论]诱导胚性愈伤组织分化为胚状体的最佳培养基为MS+2.0mg/L6-BA+0.6mg/LNAA。
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| 关 键 词： | 攸县油茶 胚状体 组织培养 组织学观察 |
| 文章编号： | 0517-6611(2008)08-03203-02 |
| 修稿时间： | 2008年1月3日 |
Study on Somatic Embryogenesis and Histological Observation of Camellia yuhsienensis Hu |
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| PENG Qiu-fa.Study on Somatic Embryogenesis and Histological Observation of Camellia yuhsienensis Hu[J].Journal of Anhui Agricultural Sciences,2008,36(8):3203-3204. |
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| Authors: | PENG Qiu-fa |
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| Abstract: | Objective] The research aimed to investigate the somatic embryogenesis from Camellia yuhsienensis Hu using cotyledons as explants. Method] Embryogenic calluses were obtained from cotyledons which had been cultured in MS 2.0 mg/L 2, 4-D 1.0 mg/L KT. Then, embryogenic calluses were incubated for somatic embryogenesis in MS basic medium with different concentrations of 6-BA and NAA. Somatic embryos at different developmental stages were sectioned and observed under light microscope. Result] The results showed that the embryogenic calluses were initiated within 15 d. The optimal medium for somatic embryo differentiation was MS 2.0 mg/L 6-BA 0.6 mg/L NAA and the induction frequency was 87.78%. The somatic embryos were also formed in the MS medium without phytohormones, but the induction frequency was less than 6.67%. The histological observations indicated that somatic embryos originated from epidermal cells or some cells near epidermis. The mature somatic embryos were finally formed via globular embryo, heart-shaped embryo and cotyledon-shaped embryo stages. Conclusion] The optimal medium for somatic embryos differentiation from embryogenic callues was MS 2.0 mg/L 6-BA 0.6 mg/L NAA. |
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| Keywords: | Camellia yuhsienensis Hu Somatic embryo In vitro Histological observations |
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