| Pb~(2+)·Cu~(2+)诱发蝌蚪红细胞微核及核异常的研究 |
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| 引用本文： | 周雪瑞,沈后方,潘正军,王丽君.Pb~(2+)·Cu~(2+)诱发蝌蚪红细胞微核及核异常的研究[J].安徽农业科学,2008,36(14):5842-5843. |
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| 作者姓名： | 周雪瑞 沈后方 潘正军 王丽君 |
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| 作者单位： | 1. 淮阴师范学院生物系,江苏淮安,223300;江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安,223300
2. 淮阴师范学院生物系,江苏淮安,223300 |
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| 基金项目： | 江苏省高校自然科学基础研究项目资助(06KJD180032). |
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| 摘 要： | 目的]研究不同浓度的Pb2+、Cu2+对蝌蚪红细胞微核率和核异常率的影响。方法]采用微核试验法,分别配制不同浓度的Pb2+(0.125～20.000 mg/L)、Cu2+(0.01～0.15 mg/L)溶液,并以经曝气后的自来水为对照进行诱发蝌蚪红细胞微核及核异常的研究。结果]毒染1周后,大于等于0.5 mg/L的Pb2+组及所有Cu2+组蝌蚪红细胞微核细胞率及核异常细胞率均大于对照组,且蝌蚪红细胞微核率及核异常率在该试验浓度范围内随离子溶液浓度的升高而增大。其中,Cu2+的毒害又明显严重于Pb2+。结论]为探讨Pb2+和Cu2+的毒害机制,及研究分析污水对水生生物的毒害机理提供了新的科学依据。
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| 关 键 词： | Pb2+ Cu2+ 红细胞 微核试验 蝌蚪 |
| 文章编号： | 0517-6611(2008)14-05842-02 |
| 修稿时间： | 2008年3月6日 |
Study on Micronuclear and Nuclear Abnormalities Induced by Pb2 and Cu2 in Tadpoles Erythrocyte |
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| ZHOU Xue-rui.Study on Micronuclear and Nuclear Abnormalities Induced by Pb2 and Cu2 in Tadpoles Erythrocyte[J].Journal of Anhui Agricultural Sciences,2008,36(14):5842-5843. |
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| Authors: | ZHOU Xue-rui |
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| Abstract: | Objective] The aim of this paper was to study the influence of Pb2 and Cu2 with different densities on tadpoles,micronuclear and nuclear abnormal rates.Method] By the method of micronucleus,different densities of solutions with Pb2 (0.125-20.000 mg/L) and Cu2 (0.01-0.15mg/L)were collocated.Taking running water out of the air as the control,study on micronuclear and the nuclear abnormality induction of erythrocyte was carried out.Result] After a week,the tadpoles abnormal rates of red blood cell micronucleus and nucleus were higher than those of control group with significant differences,except for the groups with lower than 0.5 mg/L Pb2 .And the micronucleus and nucleus abnormal rates increased with the rising of the ion solution density.Conclusion] This research provided scientific basis for the discussion on Pb2 and Cu2 toxic mechanisms and the analysis of the mechanism of polluted water toxicity on aquatic organisms. |
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| Keywords: | Pb2 Cu2 Erythrocyte Micronucleus test Tadpole |
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