| 激光共聚焦显微镜技术进展 |
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| 引用本文： | 马亢,施传信,裴冬丽,闫永峰.激光共聚焦显微镜技术进展[J].农学学报,2016,6(6):30-35. |
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| 作者姓名： | 马亢 施传信 裴冬丽 闫永峰 |
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| 作者单位： | 商丘师范学院 生命科学学院 河南商丘 476000,商丘师范学院 生命科学学院 河南商丘 476000,商丘师范学院 生命科学学院 河南商丘 476000,商丘师范学院 生命科学学院 河南商丘 476000 |
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| 基金项目： | ：国家自然科学基金“番茄MADS-box 类转录因子新基因ShMADSTF的抗病分子机制”(31571997)；河南省科技厅科技攻关项目“微生物转化法高效制备橄榄油活性成分羟基酪醇”(152102210125)。 |
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| 摘 要： | 激光共聚焦显微镜技术发展迅速，新技术主要集中于从提高分辨率、降低光毒性、提高扫描速度、活体观察等方面提升激光共聚焦显微镜的功能，各种新技术带来更多的生物学应用潜力，因此有必要梳理分析各项新技术的原理。归纳分析了光门控、白激光、GaAsP光谱检测器、超高分辨率、活细胞工作站、光片成像系统、多光子、相干反斯托克斯拉曼散射、激光捕获显微切割等技术的原理和应用潜力。并指出了现有技术在扫描速度、兼容性、样品适用性等方面的不足，展望了下一步的研究方向。该论文供研究人员在熟悉和了解新技术的基础上更加清晰的获知“基于什么样的技术能够达到什么样的生物学功能”，进而获得更多的实验思路。
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| 关 键 词： | 报废火药水解产物 报废火药水解产物 小麦 耐高温性 生理机制 |
| 收稿时间： | 2016/1/29 0:00:00 |
| 修稿时间： | 2016/4/8 0:00:00 |
The Progress of Confocal Laser Scanning Microscope |
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| Abstract: | Abstracts: At present, technique of the confocal laser scanning microscope is booming. This new technology aimed to improve the functions of the confocal laser scanning microscope from the following aspects: improve resolution, decrease the phototoxicity, and improve scanning speed, viviperception. Meanwhile, the potential of biology applications was great with the help of these new techniques, so it was necessary to sort out and analyze the principles of these new techniques. This essay mainly analyzed some techniques’ principles and potential in their applications, including Light gate, White laser, GaAsP, Ultrahigh-resolution, Live cell imaging system, Light-sheet ,Multi-photon microscopy, Coherent Anti-stokes Raman Scattering, Laser Capture Microdissection. Besides, the next research area and the shortcomings of the existing technique in scanning speed, compatibility, applicability of samples are also analyzed. This essay can assist researchers to learn more about some new techniques and make them know “what kind of technique they can use to achieve what kind of biology functions”in order that they can form more ideas of experiment research. |
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| Keywords: | Confocal laser scanning microscopes Laboratory management Large-scale precision instruments Ultrahigh-resolution |
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