| 榆黄蘑(Pleurotus citrinopileutus)原生质体分离和再生的研究 |
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| 引用本文： | 于彦春,王丕武,李玉.榆黄蘑(Pleurotus citrinopileutus)原生质体分离和再生的研究[J].吉林农业大学学报,1999,21(2):26-29. |
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| 作者姓名： | 于彦春 王丕武 李玉 |
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| 作者单位： | 吉林农业大学农学院,长春130118 |
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| 摘 要： | 试验从影响原生质体制备的诸多方面进行了研究，探索榆黄蘑原生质体制备的优化系统。试验时取鲜嫩的菌丝，用ＰＨ值为６．０的ＣＫｌ，蔗糖，甘露醇做酶解稳定液，３０℃下水浴酶解，酶解时间为４－５ｈ，此时榆黄蘑原生质体释放量最大，达到１０＾７个／ｍＬ。
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| 关 键 词： | 榆黄蘑 原生质体 分离 再生 |
| 文章编号： | 1000-5684（1999）02-0026-04 |
| 修稿时间： | 1998-12-23 |
Study on the Protoplast Isolation and Regeneration in Pleurotus citrinopileutus |
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| YU Yan-chun,WANG Pei-wu,LI Yu.Study on the Protoplast Isolation and Regeneration in Pleurotus citrinopileutus[J].Journal of Jilin Agricultural University,1999,21(2):26-29. |
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| Authors: | YU Yan-chun WANG Pei-wu LI Yu |
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| Abstract: | The experiment was researched in many ways which affect protoplast preparation. A superior system of protoplast of Pleurotus citrinopileutus preparation was studied: fresh Fungus threads were hydrolysised by lywallzyme which was dissolved in KCl or annitol or sucrose in different pH conditions.Under the conditions of T=30 and pH =6 and the time of enzyme hydrolysis during 45 h, the burst rate of protoplast was up to max. The rate of releasion isabout 107/mL. |
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| Keywords: | Pleurotus citrinopileutus protoplaste isolation regeneration |
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