| 不同水温和饲料蛋白质水平对镜鲤血清生化指标的影响 |
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| 引用本文： | 唐玲,徐奇友,王常安,尹家胜.不同水温和饲料蛋白质水平对镜鲤血清生化指标的影响[J].大连水产学院学报,2011,26(1):41-46. |
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| 作者姓名： | 唐玲 徐奇友 王常安 尹家胜 |
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| 作者单位： | 唐玲,TANG Ling(中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070;上海海洋大学,水产与生命学院,上海,201306);徐奇友,王常安,尹家胜,XU Qi-you,WANG Chang-an,YIN Jia-sheng(中国水产科学研究院黑龙江水产研究所,黑龙江,哈尔滨,150070) |
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| 基金项目： | 现代农业产业技术体系建设专项，黑龙江水产研究所基本科研专项 |
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| 摘 要： | 研究了不同水温和饲料蛋白质水平对镜鲤Cyprinus specularis血清生化指标的影响。试验中设3个温度梯度（18、22、26℃）,每个温度下投喂5种含不同蛋白质水平（质量分数为29%、31%、34%、38%、40%）的饲料,共15组,每组设3个重复,每个重复放14尾鱼,共饲喂60 d。结果表明：镜鲤血清中的肌酐（CREA）浓度随饲养水温的升高而降低,在22、26℃下CREA浓度显著低于18℃下（P〈0.05）;血清中总胆固醇（CHOL）浓度随水温的升高而升高,在22℃和26℃下CHOL浓度显著高于18℃下（P〈0.05）;血清中甘油三酯（TG）、低密度脂蛋白（LDH）、谷草转氨酶（AST）浓度和AST/ALT在22℃下比18℃和26℃下有所降低,但无显著差异（P〉0.05）。各水温环境下,血糖（GLU）、总蛋白（TP）、球蛋白（GLB）、白蛋白（ALB）浓度随饲料中蛋白质水平的增加而升高;在18℃下,LDH浓度随蛋白质水平的增加而逐渐降低,其中38%蛋白质组显著高于29%蛋白质组（P〈0.05）;在22℃下,CHOL浓度随蛋白质水平的增加而降低,其中40%蛋白质组显著低于其它蛋白质组（P〈0.05）;在26℃下,40%蛋白质组的谷丙转氨酶（ALT）活性显著高于31%和34%蛋白质组（P〈0.05）。这表明22℃下比18、26℃下更有利于镜鲤的营养代谢,18、22、26℃下,饲料中蛋白质的质量分数分别为38%、40%、40%时,有利于改善镜鲤血清生化指标。
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| 关 键 词： | 镜鲤 水温 蛋白质水平 血清生化指标 |
Effects of dietary protein levels on blood biochemical parameters in mirror common carp(Cyprinus specularis) at different temperatures |
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| TANG Ling,XU Qi-you,WANG Chang-an,YIN Jia-sheng.Effects of dietary protein levels on blood biochemical parameters in mirror common carp(Cyprinus specularis) at different temperatures[J].Journal of Dalian Fisheries University,2011,26(1):41-46. |
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| Authors: | TANG Ling XU Qi-you WANG Chang-an YIN Jia-sheng |
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| Institution: | TANG Ling1,2,XU Qi-you1,WANG Chang-an1,YIN Jia-sheng1(1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China,2.Fisheries and Life Science College,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | A feeding trial was conducted to study the effects of dietary protein levels（29%,31%,34%,38%,and 40%） on serum biochemical parameters in mirror common carp（Cyprinus specularis） with body weight of 165.24±8.07 g at different temperature（18,22,and 26 ℃）.The common carp were randomly assigned to 15 groups and fed different diets with triplication and 14 fish in each replicate for 8 weeks.The results showed creatinine（CREA） and creatine phosphokinase（CK） were found to decrease with increase in temperature,significantly lower at 22 ℃ and 26 ℃than at 18 ℃（P0.05）.There were no significant differences in triglyceride（TG）,low-density lipoprotein（LDH）,glutamate-oxaloacetate transaminase（AST）,and AST/ glutamate-pyruvate transaminase（ALT） levels at all temperature（P0.05）,and total cholesterol（CHOL） level was found to decrease significantly at 22 ℃ than at 18 ℃ and 26 ℃（P0.05）.The blood glucose（GLU）,total protein（TP）,globulin（GLB）,and albumin（ALB） were found to increase with increase in dietary protein levels.At 18 ℃,LDH level was found to increase as the dietary protein gradually decreased,significantly higher in 38% protein group than that in 29% protein group（P0.05）.At 22 ℃,CHOL was decreased as protein levels increased,significantly lower in 40% group than that in other protein groups（P0.05）.At 26 ℃,ALT was significantly higher in 40% group than that in 34% and 38% groups（P0.05）.It was concluded that common carp was considered to live better at 22 ℃ than at 18 ℃ and 26 ℃ according to serum GLU,TP,LDH,CREA,CK,CHOL and ALT levels.The dietary protein of 38%,40%,and 40% was appropriate for serum biochemical parameters in common carp at 18,22,and 26 ℃. |
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| Keywords: | mirror common carp temperature protein level serum biochemical parameter |
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