| 超低温冷冻对俄罗斯鲟精子抗氧化酶活性的影响 |
| |
| 引用本文： | 章龙珍,江琪,庄平,黄晓荣,闫文罡.超低温冷冻对俄罗斯鲟精子抗氧化酶活性的影响[J].大连水产学院学报,2009,24(6):504-508. |
| |
| 作者姓名： | 章龙珍 江琪 庄平 黄晓荣 闫文罡 |
| |
| 作者单位： | 1. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海,200090;上海海洋大学,水产与生命学院,上海,201306
2. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海,200090;上海海洋大学,水产与生命学院,上海,201306;上海市高校水产养殖学E-研究院,上海,201306 |
| |
| 基金项目： | 国家科技基础条件平台项目，国家自然科学基金资助项目，中央级公益性科研院所基本科研业务费专项资金资助项目，上海市高校水产养殖学E-研究院建设项目，浙江省科技计划项目，农业科技成果转化项目 |
| |
| 摘 要： | 为了探讨超低温（-196℃）对精子的损伤机理，采用试剂盒测定了冷冻前后俄罗斯鲟精浆和精子中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH—PX）和谷胱甘肽还原酶（GR）等酶活性。结果表明，经过超低温冷冻保存后，俄罗斯鲟精子活力下降，添加抗冻保护液冷冻后的精浆中SOD、CAT、GSH—PX活性较鲜精组显著升高（P〈0．05），较未添加保护液组显著降低（P〈0．05），GR的活性则显著低于鲜精组（P〈0．05），但显著高于未添加保护液组（P〈0．05）；添加抗冻保护液冷冻后的精子中SOD、CAT、GSH—PX活性较鲜精组显著降低（P〈0．05），较未添加保护液组显著升高（P〈0．05），GR活性则显著高于鲜精组（P〈0．05），但显著低于未添加保护液组（P〈0．05）。这说明超低温冷冻对俄罗斯鲟精子的抗氧化系统产生了较大影响，成为影响精子活力的一个重要原因。
|
| 关 键 词： | 超低温冷冻 俄罗斯鲟 精子 抗氧化酶 |
Effects of cryopreservation on antioxidant activities of semen enzymes in Russian sturgeon Acipenser gueldenstaedti |
| |
| ZHANG Long-zhen,JIANG Qi,ZHUANG Ping,HUANG Xiao-rong,YAN Wen-gang.Effects of cryopreservation on antioxidant activities of semen enzymes in Russian sturgeon Acipenser gueldenstaedti[J].Journal of Dalian Fisheries University,2009,24(6):504-508. |
| |
| Authors: | ZHANG Long-zhen JIANG Qi ZHUANG Ping HUANG Xiao-rong YAN Wen-gang |
| |
| Institution: | ZHANG Long-zhen1,2,JIANG Qi1,ZHUANG Ping1,3,HUANG Xiao-rong1,YAN Wen-gang1,2(1.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key , Open Laboratory of Marine , Estuarine Fisheries Resources , Ecology,Ministry of Agriculture,Shanghai 200090,China,2.College of Marine Sciences,Shanghai Ocean University,Shanghai 201306,3.Aquaculture Division,E-Institute of Shanghai Universites,China) |
| |
| Abstract: | The effects of cryopreservation(-196 ℃)on the antioxidant activities of enzymes (SOD, CAT, GSH-PX and GR) in seminal plasma and spermatozoa of Russian sturgeon Acipenser gueldenstaedti were studied by a reagent kit to clarify damage mechanism of spermatozoa. The spermatozoa were found to show lower motility after preserved compared to the fresh semen, and the activities of SOD,CAT,GSH-PX in seminal plasma supplemented with cryoprotectants increased significantly (P<0.05)compared to the control group (P<0.05). The activity of GR showed significantly lower compared to the fresh seminal plasma(P<0.05),but significantly higher compared to the seminal plasma without supplementation of the cryoprotectants (P<0.05).Compared to the fresh sperm, the spermatozoa supplementation of the cryoprotectants had lower activities of SOD,CAT,GSH-PX(P<0.05).However, the significantly higher activity of GR was observed in the spermatozoa supplementation of the cryoprotectants compared to the fresh sperm(P<0.05),and the significantly lower activity of GR was found in the seminal plasma without supplementation of the cryoprotectants (P<0.05). The results showed that cryopreservation had significant effects on anti-oxidative system in the spermatozoa of Russian sturgeon and the motility of the spermatozoa. |
| |
| Keywords: | cryopreservation Acipenser gueldenstaedti semen antioxidant enzyme |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
