| 4株禽腺病毒的鉴定和分析 |
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| 作者姓名： | 胡晓苗 潘孝成 张丹俊 赵瑞宏 戴银 周学利 侯宏艳 沈学怀 尹磊 |
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| 作者单位： | 安徽省农业科学院畜牧兽医研究所;安徽省畜禽疫病研究中心;畜禽产品安全工程安徽省重点实验室 |
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| 基金项目： | 安徽省农科院平台项目(2019YL065);安徽省农科院科研团队项目(2019YL076) |
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| 摘 要： | 目的]对六安、郭河、宣城、肥西某养殖户送检的疑似腺病毒的病料进行分离鉴定,为进一步鉴别、预防和控制疾病提供科学的理论依据。方法]首先采集病例中鸡的肝脏,提取病毒DNA,根据Genbank已登录腺病毒的hexon基因设计引物,通过PCR检测病料中腺病毒,对扩增的序列进行连接转化和阳性克隆子筛选,并对扩增到的序列进行测序鉴定,最后将获得的基因序列构建进化树分析确定安徽省部分地区禽腺病毒血清型。结果]临床中疑似腺病毒感染的主要症状为心包积液和肝脏肿大,用设计的引物均检测出大小为599 bp的目的基因条带,序列比对结果发现,此次检测的腺病毒均为4型腺病毒。结论]试验建立了快速检测禽腺病毒的方法,为安徽省地区禽腺病毒防控提供了理论依据。
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| 关 键 词： | 禽腺病毒 HEXON 测序 血清型鉴定 |
sis of Four Avian Adenovirus Strains |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031) |
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| Abstract: | Objective] The suspected adenovirus samples from a farmer in Lu’an, Guohe, Xuancheng and Feixi were isolated and identified, which provided a scientific theoretical basis for further identification, prevention and control of diseases. Method] Firstly,the chicken liver was collected, the virus DNA was extracted, and the primers were designed according to the hexon gene of the adenovirus that GenBank had registered. The adenovirus was detected by PCR, the amplified sequence was transformed and the positive clone was screened, and the amplified sequence was sequenced and identified.Finally, the obtained gene sequence was constructed and analyzed by evolutionary tree to determine the fowl adenovirus serotype in Anhui Province. Result] In clinical practice, the main symptoms of adenovirus infection were pericardial effusion and liver enlargement. The designed primers were used to detect the target gene band with a size of 599 bp.Sequence comparison results showed that all the tested adenoviruses were type 4 adenoviruses. Conclusion] The method of rapid detection of avian adenovirus was established, which provided a theoretical basis for the prevention and control of avian adenovirus in Anhui Province. |
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| Keywords: | Avian adenovirus hexon Sequencing Serotype identification |
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