| 尼罗罗非鱼致病性嗜水气单胞菌的分离鉴定与药敏试验 |
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| 引用本文： | 董忠典,周芬娜,袁颜颜,冯艳艳,王慧.尼罗罗非鱼致病性嗜水气单胞菌的分离鉴定与药敏试验[J].东北农业大学学报,2012,43(6):110-115. |
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| 作者姓名： | 董忠典 周芬娜 袁颜颜 冯艳艳 王慧 |
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| 作者单位： | 山东农业大学动物科技学院,山东泰安,271018 |
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| 基金项目： | 山东省博士后创新基金，山东省自然科学基金，山东农业大学青年创新基金 |
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| 摘 要： | 从自然患病的尼罗罗非鱼(Oreochromis niloticus)肝脏组织分离出2株细菌,命名为M-O1和M-02。用M-O1和M-02分别在实验室人工感染健康的尼罗罗非鱼,被感染鱼3周内全部死亡,且患病症状与自然发病症状一致。从被感染的鱼体中分离得到2株类似细菌(分别命名为W-01、W-02),说明分离的菌株为本次尼罗罗非鱼出血性败血症的病原菌。通过生理生化方法进行种属鉴定,确认以上分离到的4株细菌均为嗜水气单胞菌(Aeromonaz hydrophila),分别命名为M-O1、M-02、W-01、W-02。用19种常见药物对上述菌株进行药敏试验,4菌株对丁胺卡那、氟哌酸、庆大霉素、链霉素、恩诺沙星、新霉素、氟罗沙星和复方新诺明极为敏感;对氧哌嗪青霉素、利福平、麦迪霉素、红霉素、罗红霉素和阿奇霉素敏感,对羧苄青霉素、头孢噻吩、先锋霉素、氨苄青霉素和林可霉素为不敏感。
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| 关 键 词： | 尼罗罗非鱼 败血症 嗜水气单胞菌 药敏试验 |
Oreochromis niloticus pathogenic Aeromonas hydrophila isolation,identification and susceptibility testing |
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| DONG Zhongdian
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ZHOU Fenna
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YUAN Yanyan
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FENG Yanyan
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WANG Hui.Oreochromis niloticus pathogenic Aeromonas hydrophila isolation,identification and susceptibility testing[J].Journal of Northeast Agricultural University,2012,43(6):110-115. |
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| Authors: | DONG Zhongdian ZHOU Fenna YUAN Yanyan FENG Yanyan WANG Hui |
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| Institution: | (School of Animal Science and Technology,Shandong Agricultural University,Tai’an Shandong 271018,China) |
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| Abstract: | In this study,we isolated two bacterial strains,M-O1 and M-02 from liver of seriously diseased Oreochromis niloticus.The two bacterial strains M-O1 and M-02 were used to infect healthy Oreochromis niloticus,all infected fish died in three weeks,and the symptoms of the infected fish were consistent with the natural diseased fish.The results showed that M-O1 and M-02 were the right sepsis pathogens.The two bacterial strains isolated from the infected fish were named as W-01 and W-02.We confirmed these four bacterial strains were Aeromonas hydrophil by species identification experiment with physiological and biochemical methods.Susceptibility testing showed that,among 19 common antibiotics,amikacin,norfloxacin,gentamicin,streptomycin,enrofloxacin,neomycin,fleroxacin and sinomin compositea,had significant inhibition on the four bacterial strains. |
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| Keywords: | Oreochromis niloticus haemorrhagic septicaemia Aeromonas hydrophil drug test |
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