| 谷胱甘肽转移酶标签蛋白单克隆抗体的制备 |
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| 引用本文： | 李桂伟,乔薪瑗,李海滨,李一经.谷胱甘肽转移酶标签蛋白单克隆抗体的制备[J].东北农业大学学报,2008,39(2):231-234. |
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| 作者姓名： | 李桂伟 乔薪瑗 李海滨 李一经 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030 |
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| 基金项目： | 基金项目：黑龙江省“十五”科技攻关项目（GC01B510） |
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| 摘 要： | 以纯化的谷胱甘肽转移酶标签蛋白(GST)免疫BALB/c小鼠,按常规方法进行细胞融合,用纯化的GST筛选,最终获得一株抗GST的杂交瘤细胞系(命名为1D10),染色体平均记数为90对,间接ELISA检测1D10细胞培养上清的效价为1:4 000。腹水效价为1:5×107,该单抗可以特异识别GST蛋白和带有GST标签(GST-tagged)的融合蛋白。
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| 关 键 词： | GST 单克隆抗体 间接ELISA |
| 文章编号： | 1005-9369（2008）02-0231-04 |
| 收稿时间： | 2007-01-05 |
| 修稿时间： | 2007年1月5日 |
Production of monoclonal antibodies against GST |
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| LI Guiwei,QIAO Xinyuan,LI Haibin,LI Yijing.Production of monoclonal antibodies against GST[J].Journal of Northeast Agricultural University,2008,39(2):231-234. |
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| Authors: | LI Guiwei QIAO Xinyuan LI Haibin LI Yijing |
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| Abstract: | The monoclonal antibody(McAb) against GST was performed by immunizing the BABL/c mice with purified GST.Cells fusion was carried out by standard method and hybridmas secreting antibody were subcloned three times with limiting dilution.One hybridoma cell line named 1D10 was picked out by indirect ELISA,which secreted monoclonal antibody specific against GST,and its average number of chromosomes was 90 paries.The antibody titer of cell culture supernatant and ascite were 1:4 000 and 1:5×107,respectively. |
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| Keywords: | GST |
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