| 改良CTAB法提取益智基因组DNA |
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| 引用本文： | 刘晓静,王海燕,张根良,夏志强,王文泉.改良CTAB法提取益智基因组DNA[J].江西农业学报,2007,19(8):111-112,114. |
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| 作者姓名： | 刘晓静 王海燕 张根良 夏志强 王文泉 |
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| 作者单位： | 1. 华南热带农业大学,农学院,海南,儋州,571737;中国热带农业科学院,生物技术所,海南,海口,571101
2. 中国热带农业科学院,生物技术所,海南,海口,571101 |
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| 摘 要： | 益智叶片中多酚和多糖等杂质的含量较高,为获得高质量的益智基因组DNA,试验在传统CTAB法的基础上加以改良,提取益智基因组DNA,并对所得DNA的质量和浓度进行了检测。结果表明,改良后的方法较之传统方法简单高效,所获得的基因组DNA质量较好,OD260/OD280在1.7~2.0之间,RNA消化彻底,DNA无明显降解,进行ISSR分析条带清晰,多态性好。适合于进行以PCR为基础的DNA多态性的研究。
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| 关 键 词： | 益智 基因组DNA 改良CTAB法 |
| 文章编号： | 1001-8581（2007）08-0111-02 |
| 收稿时间： | 2007-05-13 |
| 修稿时间： | 2007-05-13 |
Improved CTAB Method for Extraction of Genome DNA from Alpinia oxyphylla Miq. |
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| LIU Xiao-jing,WANG Hai-yan,ZHANG Gen-liang,XIA Zhi-qiang,WANG Wen-quan.Improved CTAB Method for Extraction of Genome DNA from Alpinia oxyphylla Miq.[J].Acta Agriculturae Jiangxi,2007,19(8):111-112,114. |
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| Authors: | LIU Xiao-jing WANG Hai-yan ZHANG Gen-liang XIA Zhi-qiang WANG Wen-quan |
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| Institution: | 1. Collage of Agronomy, South China University of Tropical Agriculture, Danzhou, 571737, China; 2. Institute of Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,Haikou 571101 ,China |
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| Abstract: | Genome DNA was extracted by the improved CTAB method from leaves of Alpinia oxyphylla Miq.which was rich in polysaccharide and polyphenol,and the quality and concentration of DNA were detected.The results indicated that improved CTAB method was a simple and high efficient method which could obtain the high quality of genome DNA,the value of OD260/OD280 was 1.7 to 2.0,the RNA was eliminated completely without obvious degradation of DNA,and then ISSR analysis could produced clear polymorphic patterns.Therefore this method was suitable for the polymorphic analysis based on PCR. |
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| Keywords: | Alpinia oxyphylla Miq Genome DNA Improved CTAB method |
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