| EMS与NaN_3对甘蓝型油菜和芥菜型油菜诱变的效果 |
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| 引用本文： | 赵福永,郑娇,何芳,何文俊,李利露.EMS与NaN_3对甘蓝型油菜和芥菜型油菜诱变的效果[J].江西农业学报,2010,22(9). |
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| 作者姓名： | 赵福永 郑娇 何芳 何文俊 李利露 |
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| 作者单位： | 长江大学,生命科学学院,湖北,荆州,434025 |
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| 基金项目： | 长江大学博士启动基金 |
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| 摘 要： | 采用化学诱变剂甲基磺酸乙酯(EMS)和叠氮化钠(NaN3)分别以不同浓度和处理时间组合,对3个甘蓝型油菜品种(湘油15号、中油821、中双9号)和3个芥菜型油菜品种(金油王2号、品系153和154)成熟种子进行诱变。以未处理组为对照,比较了诱变当代(M1)种子的发芽率和子代(M2)株高、叶色、花色、种皮颜色和花粉育性等生物学性状。结果发现,随着诱变剂浓度的增加和处理时间的延长各品种(系)M1代种子的发芽率逐渐降低,且相同处理条件下芥菜型油菜的平均发芽率要低于甘蓝型油菜;在M2代中初步筛选出28个甘蓝型油菜和38个芥菜型油菜的株高、叶色、花色等性状的突变体,为今后油菜遗传改良和功能基因组学研究提供了更丰富的研究材料。
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| 关 键 词： | 油菜 突变体 诱变 甲基磺酸乙酯(EMS) 叠氮化钠(NaN3) |
Research on Mutagenic Effect of EMS and NaN3 on Brassica napus and Brassica juncea |
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| ZHAO Fu-yong,ZHENG Jiao,HE Fang,HE Wen-jun,LI Li-lu.Research on Mutagenic Effect of EMS and NaN3 on Brassica napus and Brassica juncea[J].Acta Agriculturae Jiangxi,2010,22(9). |
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| Authors: | ZHAO Fu-yong ZHENG Jiao HE Fang HE Wen-jun LI Li-lu |
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| Institution: | ZHAO Fu-yong,ZHENG Jiao,HE Fang,HE Wen-jun,LI Li-lu(College of Life Science,Yangtze University,Jingzhou 434025,China) |
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| Abstract: | In this study,the mature seeds of three Brassica napus cultivars(Xiangyou No.15,Zhongyou 821 and Zhongshuang No.9) and three Brassica juncea varieties(Jinyouwang No.2,Line 153 and Line 154) were induced by Ethyl Methan Sulfonate(EMS) and Sodium azide(NaN3) with different combinations of concentration and treatment time.Using the untreated group as the control group,the germination rate of seeds in generation M1 and the biological traits such as plant height,leaf color,flower color,seed color and pollen fert... |
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| Keywords: | Rapeseed Mutant Ethyl Methan Sulfonate(EMS) Sodium azide(NaN3) |
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