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| 湖羊GnRH受体基因的单核苷酸多态性研究 | |
| 引用本文： | 刘源,应诗家,祝铁钢,王锋,王子玉,张有法,陈琳,石国庆,张永胜.湖羊GnRH受体基因的单核苷酸多态性研究[J].江西农业学报,2009,21(7):4-8. |
| 作者姓名： | 刘源 应诗家 祝铁钢 王锋 王子玉 张有法 陈琳 石国庆 张永胜 |
| 作者单位： | 1. 南京农业大学,动物胚胎工程技术中心,江苏,南京,210095 2. 苏州市种羊场,江苏,苏州215125 3. 新疆兵团绵羊繁育生物技术重点实验室,新疆,石河子,832000 4. 新疆紫泥泉绵羊育种中心,新疆,石河子,832025 |
| 基金项目： | 国家科技支撑计划项目 |
| 摘 要： | 对高繁殖力绵羊品种(湖羊)和低繁殖力绵羊品种(陶赛特、特克赛尔和哈萨克)促性腺激素释放激素受体(GnRH-R)基因的5′非翻译区、外显子1和外显子2部分序列进行了PCR-SSCP分析,结果发现在5′非翻译区发生3处碱基突变(552T→G、653C→G、654G→A);在第二外显子区发生1处碱基突变(230G→C),该突变导致了第66位氨基酸的改变(精氨酸→丝氨酸)。并对这4个位点在4个绵羊品种间的基因频率进行了初步分析:对于引物1,4个绵羊群体均检测到AA基因型,AB基因型只出现在湖羊、陶赛特羊和特克塞尔羊中,仅在哈萨克羊中检测到CC基因型。对于引物2,4个绵羊群体均检测到DD基因型;DE基因型只出现在湖羊和哈萨克羊中;仅在湖羊中检测到EE基因型。 |
| 关 键 词： | 促性腺激素释放激素受体(GnRH-R)基因 5′非翻译区 单核苷酸多态性 PCR-SSCP |
Single Nucleotide Polymorphisms of GnRH Receptor Gene in Hu Sheep |
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| LIU Yuan,YING Shi-jia,ZHU Tie-gang,WANG Feng,WANG Zi-yu,ZHANG You-fa,CHEN Lin,SHI Guo-qing,ZHANG Yong-sheng.Single Nucleotide Polymorphisms of GnRH Receptor Gene in Hu Sheep[J].Acta Agriculturae Jiangxi,2009,21(7):4-8. | |
| Authors: | LIU Yuan YING Shi-jia ZHU Tie-gang WANG Feng WANG Zi-yu ZHANG You-fa CHEN Lin SHI Guo-qing ZHANG Yong-sheng |
| Institution: | 1.Center of Animal Embryo Engineering & Technology;Nanjing Agricultural University;Nanjing 210095;China;2.Breeding Sheep Farm of Suzhou City in Jiangsu Province;Suzhou 215125;3.Key Laboratory for Biotechnology of Sheep Breeding of Xinjiang Production & Construction Corps;Shihezi 832000;4.Ziniquan Research Institute of Sheep Breeding;Shihezi 832025;China |
| Abstract: | Single nucleotide polymorphisms(SNPs) of GnRH receptor gene 5′ notranslated region,exon 1 and 2 were detected in high fecundity breed(Hu sheep) and low fecundity breeds(Dorset,Texel and Kazakstan sheep) with two pairs of primers by PCR-SSCP.Sequencing revealed three mutations(552T→G,653C→G,654G→A) in 5′ notranslated region,and a mutation(230 G→C) in exon 2 which resulted in the change of amino acids(66 Arg→Ser).The gene frequencies of 4 sites in four breeds were analyzed.For primer 1,AA genotype was detected in four sheep populations,AB genotype was detected in Hu,Dorset and Texel sheep,CC genotype was only detected in Kazakstan sheep.For primer 2,DD genotype was detected in four sheep populations,DE genotype was detected in Hu and Kazakstan sheep,EE genotype was only detected in Hu sheep. |
| Keywords: | PCR-SSCP |
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