| 饲料中添加木聚糖酶对刺参幼参生长、消化和体腔液酶活力的影响 |
| |
| 引用本文： | 王际英,魏佳丽,孙永智,李宝山,张利民,李培玉,王雅平,武明欣.饲料中添加木聚糖酶对刺参幼参生长、消化和体腔液酶活力的影响[J].大连海洋大学学报,2018(3):329-335. |
| |
| 作者姓名： | 王际英 魏佳丽 孙永智 李宝山 张利民 李培玉 王雅平 武明欣 |
| |
| 作者单位： | 1. 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室,山东 烟台,264006;2. 上海海洋大学 水产科学国家级实验教学示范中心,上海201306;3. 上海海洋大学上海水产养殖工程技术研究中心,上海201306;4. 上海海洋大学农业部鱼类营养与环境生态研究中心,上海201306 |
| |
| 基金项目： | 山东省现代农业产业技术体系刺参产业创新团队项目(SDAIT-22-06)，山东省重点研发计划项目(2016GSF115005)，烟台市科技发展计划项目(2015ZH075) |
| |
| 摘 要： | 为探讨木聚糖酶添加量对刺参Apostichopus japonicus生长、体成分、消化酶和体腔液酶活力的影响,在饲料中添加不同水平(0、0.03%、0.06%、0.09%、0.12%和0.24%)的木聚糖酶制成6种等氮等能试验饲料,试验设6组,每组设3个平行,每个平行放体质量为(7.73±0.09)g的幼参50头,分别用6种饲料进行投喂,共进行56 d养殖试验。结果表明:饲料中添加木聚糖酶显著提高了幼参增重率和特定生长率(P0.05),且0.09%木聚糖酶添加组显著高于其他各组(P0.05),各添加组增重率与对照组相比均显著提高(P0.05);木聚糖酶添加组幼参体壁粗蛋白质含量显著高于对照组(P0.05),0.06%~0.24%木聚糖酶添加组幼参粗脂肪含量显著高于对照组(P0.05);0.06%~0.12%木聚糖酶添加组肠道蛋白酶活力显著高于对照组(P0.05),0.24%添加组淀粉酶活力显著高于其他各组(P0.05);幼参体腔液中溶菌酶、过氧化氢酶、谷草转氨酶和谷丙转氨酶活力均随木聚糖酶添加量的增加呈先升高后降低的趋势,0.06%~0.12%添加组显著高于对照组(P0.05)。研究表明,饲料中添加木聚糖酶可显著提高刺参生长、体成分、消化酶和体腔液酶活力,以增重率为评价指标,折线回归分析得出,对平均体质量为(7.73±0.09)g的刺参,其饲料中木聚糖酶最适添加量为900 mg/kg。
|
| 关 键 词： | 刺参 木聚糖酶 生长 消化 免疫酶活力 Apostichopus japonicus xylanase growth digestion immune enzyme activity |
Effects of dietary xylanase on growth,digestion and immune enzyme activity in sea cucumber Apostichopus japonicus |
| |
| Authors: | WANG Ji-ying WEI Jia-li SUN Yong-zhi LI Bao-shan ZHANG Li-min LI Pei-yu WANG Ya-ping WU Ming-xin |
| |
| Abstract: | A 56-day feeding trial was conducted to evaluate the effect of xylanase on growth performance, body composition,digestive enzyme activity and immune enzyme activity of sea cucumber with initial body weight of (7.73±0.09)g. Juvenile sea cucumber were reared in cylindrical fiber glass tanks (70 cm×80 cm) at a density of 50 individuals per tank and fed six isonitrogenous and isoenergergetic diets containing 0,0.03%,0.06%,0.09%, 0.12% and 0.24% xylanase with 3 replicates at water temperature of(19±1)℃. The results showed that there was significantly higher growth performance in the sea cucumber fed diets containing xylanase than that in the other groups(P<0.05),significantly higher weight gain rate (WGR) in the sea cucumber fed diets containing 0.03%-0.24% xylanase compared to control group(P<0.05).The sea cucumber fed the diet containing xylanase had signif-icantly higher crude protein in body wall than the sea cucumber in the control group did(P<0.05). There was sig-nificantly higher crude lipid content in the sea cucumber in 0.06%-0.24% xylanase groups than that in the control group(P<0.05). The sea cucumber fed the diet containing xylanase at a rate of 0.06%-0.12% had significantly higher intestinal trypsin activities than the sea cucumber in the control group did(P<0.05). Amylase activity was found to be significantly higher in the 0.24% xylanase group than that in the other groups(P<0.05). The activities of lysozyme (LZM),catalase (CAT), glutamic-oxalacetic transaminase (AST), glutamic-pyruvic transaminase (ALT) and superoxide dismutase (SOD) were shown an increasing tendency first and then decrease with the in-creasing dietary xylanase, significantly higher LZM, and CAT activities in coelomic fluid of sea cucumber fed the diet containing xylanase at dose of 0.06%-0.12%(P<0.05). The findings indicate that the addition of xylanase leads to improve growth performance,body composition,digestive enzyme activity and immunity capabilities of sea cucumber,and that supplementation of xylanase at a dose of 900 mg/kg is of the optimal level for sea cucumber by Broken-line regression of WGR. |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
