| β-甘露聚糖酶分子生物学研究进展 |
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| 引用本文： | 张蕊,朱虹,周峻沛,黄遵锡.β-甘露聚糖酶分子生物学研究进展[J].中国农业科技导报,2018,20(5):34-46. |
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| 作者姓名： | 张蕊 朱虹 周峻沛 黄遵锡 |
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| 作者单位： | 云南师范大学生命科学学院, 生物能源持续开发利用教育部工程中心, 昆明 650500 |
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| 基金项目： | 国家自然科学基金项目(31460694)；云南师范大学2016年大学生科学研究训练基金项目(ky2016-134)资助。 |
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| 摘 要： | β-甘露聚糖酶在食品、饲料、造纸、洗涤等工业领域应用广泛。近几年,基因测序技术飞跃发展,研究人员挖掘到许多新颖的β-甘露聚糖酶基因并对其进行了酶学性质的研究。介绍了各种类型的甘露聚糖及其降解酶,梳理了β-甘露聚糖酶的糖苷水解酶家族分类、来源、结构和催化机理,归纳总结了近几年微生物来源β-甘露聚糖酶的重组表达、酶学性质及分子改造,简述了甘露聚糖酶在食品和饲料等方面的应用,展望了β-甘露聚糖酶的研究热点及方向。
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| 关 键 词： | &beta -甘露聚糖酶 糖苷水解酶 重组表达 酶学性质 分子改造 |
Research Progress on Molecular Biology of β-mannanases |
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| ZHANG Rui,ZHU Hong,ZHOU Junpei,HUANG Zunxi.Research Progress on Molecular Biology of β-mannanases[J].Journal of Agricultural Science and Technology,2018,20(5):34-46. |
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| Authors: | ZHANG Rui ZHU Hong ZHOU Junpei HUANG Zunxi |
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| Institution: | Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education;
School of Life Science, Yunnan Normal University, Kunming 650500, China |
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| Abstract: | Endo-β-mannanase is widely applied in various industries including food, feed, paper making and lavation. Recently, with the overflying development of gene sequencing technology, many novel endo-β-mannanases have been unearthed and their enzymatic properties have been investigated in recent year. The paper introduced various types of mannan and its degrading enzymes; summarized classification, sources, structures and catalytic mechanisms of endo-β-mannanases; and summarized the recombination expression, enzymatic properties and molecular modification of endo-β-mannanases sourced from microorganisms in recent years. Furthermore, the paper briefly described the application of endo-β-mannanases in food and feed industries and prospected the research hotspot and direction of endo-β-mannanase. |
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| Keywords: | β-mannanase glycoside hydrolase recombination expression enzymztic property molecular modification |
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