| 利用透明颤菌血红蛋白在低氧条件下提高毕赤酵母中植酸酶的表达 |
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| 引用本文： | 唐辉桂,黄火清,罗会颖,王亚茹,杨培龙,姚斌.利用透明颤菌血红蛋白在低氧条件下提高毕赤酵母中植酸酶的表达[J].中国农业科技导报,2008,10(3):84-89. |
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| 作者姓名： | 唐辉桂 黄火清 罗会颖 王亚茹 杨培龙 姚斌 |
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| 作者单位： | (中国农业科学院饲料研究所, 北京 100081） |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了提高毕赤酵母(Pichia pastoris)中重组植酸酶的表达,在来源于Pichia stipitis的低氧诱导启动子PsADH2控制下,进行了透明颤菌血红蛋
白基因vgb在重组P. pastoris中与植酸酶的共表达研究。结果表明,PsADH2启动子在低氧条件下被诱导启动,表达的血红蛋白VHb对P. pastoris
宿主细胞生长无明显影响,但使重组P. pastoris中共表达的植酸酶APPA活性提高了3倍。
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| 关 键 词： | PsADH2启动子 透明颤菌血红蛋白VHb 低氧诱导 植酸酶 毕赤酵母 |
| 收稿时间： | 2008-01-27 |
| 修稿时间： | 2008-05-23 |
Expression of Bacterial Hemoglobin with a Low O2-induced Promoter
Improves Recombinant Phytase Production in Pichia pastoris |
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| TANG Hui-gui,HUANG Huo-qing,LUO Hui-ying,WANG Ya-ru,YANG Pei-long,YAO Bin.Expression of Bacterial Hemoglobin with a Low O2-induced Promoter
Improves Recombinant Phytase Production in Pichia pastoris[J].Journal of Agricultural Science and Technology,2008,10(3):84-89. |
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| Authors: | TANG Hui-gui HUANG Huo-qing LUO Hui-ying WANG Ya-ru YANG Pei-long YAO Bin |
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| Institution: | (Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | To improve the production of a recombinant phytase APPA in Pichia pastoris under O2 limitation, the PsADH2 promoter from
Pichia stipitis was employed to control the expression of Vitreoscilla hemoglobin (VHb) encoding gene (vgb) in P. pastoris.
This promoter was activated under O2 limitation in P. pastoris. The expressed VHb enhanced the phytase APPA activity to 3
fold higher than that of VHb-free cell under O2 limitation, but no obvious effect on cell growth was observed. |
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| Keywords: | PsADH2-promoter Vitreoscilla hemoglobin (VHb) oxygen-regulated expression phytase P pastoris |
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