| 猪细小病毒VP2融合IFN-γ提高其在小鼠体内免疫原性 |
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| 引用本文： | 张雪花,华涛,陆吉虎,梅梅,张道华,侯继波,唐应华.猪细小病毒VP2融合IFN-γ提高其在小鼠体内免疫原性[J].中国农业科技导报,2017,19(2):35-42. |
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| 作者姓名： | 张雪花 华涛 陆吉虎 梅梅 张道华 侯继波 唐应华 |
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| 作者单位： | 1.江苏省农业科学院国家兽用生物制品工程技术研究中心, 农业部兽用生物制品工程技术重点实验室,;
南京210014; 2.江苏省动物重要疫病与人兽共患病防控协同创新中心, 江苏 扬州 225009 |
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| 基金项目： | 中央财政农业技术推广资金项目[TG(14)067]，农业部公益性行业（农业）科研专项（201303046），江苏省农业科技自主创新项目[CX(15)1063]资助。 |
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| 摘 要： | 为了检验细胞因子IFN-γ与猪细小病毒VP2融合蛋白的免疫效力,通过重叠延伸PCR技术将IFN-γ与PPV的VP2基因融合到一起,利用杆状病毒表达系统构建了重组杆状病毒r Bac-VP2、r Bac-VP2-IFN-γ,并在小鼠上验证了免疫原性。猪细小病毒ELISA抗体和中和抗体检测结果显示,VP2-IFN-γ组明显高于VP2和PPV灭活苗组;淋巴细胞增殖试验结果表明,VP2-IFN-γ组淋巴细胞数明显高于不加IFN-γ组。上述结果表明IFN-γ可提高猪细小病毒亚单位疫苗体液和细胞免疫应答,为重组IFN-γ的猪细小病毒病亚单位疫苗开发提供理论依据。
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| 关 键 词： | 猪细小病毒 VP2 IFN-&gamma 重组杆状病毒 免疫原性 |
Enhanced Immunogenicity of VP2 of Porcine Parvovirus Fused with Porcine Interferon γ in Mice |
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| ZHANG Xuehua,HUA Tao,LU Jihu,MEI Mei,ZHANG Daohua,HOU Jibo,TANG Yinghua.Enhanced Immunogenicity of VP2 of Porcine Parvovirus Fused with Porcine Interferon γ in Mice[J].Journal of Agricultural Science and Technology,2017,19(2):35-42. |
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| Authors: | ZHANG Xuehua HUA Tao LU Jihu MEI Mei ZHANG Daohua HOU Jibo TANG Yinghua |
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| Institution: | 1.Key Laboratory of Engineering and Technology for Veterinary Blologicals, Ministry of Agriculture; National Research ;
Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, ;
Nanjing 210014; 2.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious ;
Diseases and Zoonoses, Jiangsu Yangzhou 225009, China |
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| Abstract: | In order to identify the immunogenicity of porcine parvovirus VP2 fused with porcine interferon γ(IFN-γ), VP2 of PPV was fused with IFN-γ via overlapping extension polymerase chain reaction technique, and rBac-VP2, rBac-VP2-IFN-γ were constructed by baculoviruses expression vector system. The immunogenicity of the recombinant baculoviruses expressing VP2 of PPV fused with or without IFN-γ (rBac-VP2-IFN-γ and rBac-VP2) was characterized in BALB/c mice, and set the inactivated PPV vaccine as the control. The level of ELISA antibodies against VP2 and neutralizing antibodies against PPV, and the magnitude of lymphocyte proliferation from the mice immunized with rBac-VP2-IFN-γ vaccine were significantly higher than those of mice shot with rBac-VP2 vaccine or inactivated PPV vaccine. Together, this study showed that IFN-γ could improve the cellular and humoral immune response of subunit vaccine of PPV. This result has provided theoretical basis for the development of PPV subunit vaccine with porcine IFN-γ. |
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| Keywords: | porcine parvovirus VP2 IFN-γ recombinant baculovirus immunogenicity |
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