| 黄金鲫内脏类结节病的病原鉴定与药物敏感性试验分析 |
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| 引用本文： | 王永杰,陈红莲,程云生,王芬.黄金鲫内脏类结节病的病原鉴定与药物敏感性试验分析[J].中国农业科技导报,2017,19(12):104-109. |
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| 作者姓名： | 王永杰 陈红莲 程云生 王芬 |
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| 作者单位： | 安徽省农业科学院水产研究所, 合肥 230031 |
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| 基金项目： | 安徽省农业科学院科技创新团队项目(14C0504);安徽省水产产业技术体系专项(2016-84)资助。 |
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| 摘 要： | 淡水养殖鱼类结节病是近年来时常爆发的疾病之一,为了有效预防和防治该病,从合肥郊区某养殖场濒死的黄金鲫(鲤鱼♀×鲫鱼♂)体内分离优势致病菌(命名为AH菌株),对其进行了鉴定。采用传统细菌分类方法,测定该菌株的形态特征、生理生化特性和常见药物的敏感性;应用分子生物学方法,对病原菌16S rRNA特异片段进行克隆、序列测定分析。AH菌株与对照鮰爱德华氏菌在形态特征和生理生化特性基本一致。序列分析结果显示分离的AH菌株与鮰爱德华氏菌(AB050826,NR_024769)菌株间基因序列同源性为99.9%以上,在系统发育树上与鮰爱德华氏菌聚为一族。人工回归复感染健康黄金鲫鱼,出现与原发性相似的内脏类结节病症状。药敏实验结果显示:AH菌株对氧氟沙星等11种药物敏感,对四环素等4种抗生素耐药,对磺胺类药物复方新诺明中度敏感。综合分析认为鮰爱德华氏菌是黄金鲫内脏类结节病的重要致病菌,该结果为黄金鲫内脏类结节病有效防控提供有益参考。
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| 关 键 词： | 黄金鲫鱼 病原菌分离鉴定 药敏试验 复感染试验 同源分析 |
Isolation,Identification and Drug Sensitivity Analysis
of Gold Carp Visceral Sarcoidosis of Pathogen |
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| WANG Yongjie,CHEN Honglian,CHENG Yunsheng,WANG Fen.Isolation,Identification and Drug Sensitivity Analysis
of Gold Carp Visceral Sarcoidosis of Pathogen[J].Journal of Agricultural Science and Technology,2017,19(12):104-109. |
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| Authors: | WANG Yongjie CHEN Honglian CHENG Yunsheng WANG Fen |
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| Institution: | Institute of Fisheries, Anhui Academy of Agricultural Sciences, Hefei 230031 |
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| Abstract: | In recent years, sarcoidosis is one of the frequent outbreak disease in freshwater fishery. In order to effectively prevent and control this disease, the experiment separated the dominant pathogens (named AH strain) from the dying gold crucian carp at a fish farm of Hefei outskirts and carried out identification. The morphological characteristics, physiological and biochemical characteristics of the strain, and the sensitivity of common drugs were determined by traditional bacteria classification method, and molecular biology method was also used to clone and sequence the specific fragment of 16S rRNA. The AH strain was consistent with the control Edwards strain on the morphological characteristics and physiological biochemical characteristics. The result of sequence analysis on AH strain and catfish Edward siella strains (AB050826, NR_024769) showed that the homology to each other was over 99.9%, and they were assigned to the same strains family. After artificial infection test, similar natural incidence of visceral sarcoidosis symptoms were appeared. The results of drug sensitivity test showed that AH strain was sensitive to ofloxacin and the other 10 kinds of drugs; moderately sensitive to the sulfa drugs, such as sulfamethoxazole; and resistant to 4 kinds of antibiotics. Comprehensive analysis indicated that Edward bacteria was a virulent pathogen of gold crucian carp visceral sarcoidosis. This study provided a useful reference for the effective prevention and control of golden crucian carp offal sarcoidosis. |
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| Keywords: | gold crucian carp isolation and identification of pathogenic bacteria drug sensitivity test multiple infection test phylogenetic tree analysis |
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