| 新疆焉耆县不同动物源耐药沙门氏菌的MLST分析 |
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| 引用本文： | 王凯,林亚军,王舒丰,李淑娴,向志宇,夏利宁.新疆焉耆县不同动物源耐药沙门氏菌的MLST分析[J].中国农业科技导报,2020,22(6):111-122. |
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| 作者姓名： | 王凯 林亚军 王舒丰 李淑娴 向志宇 夏利宁 |
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| 作者单位： | 新疆农业大学动物医学学院, 乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金项目（31860714）。 |
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| 摘 要： | 为了解新疆焉耆县不同动物源沙门氏菌（Salmonella）对临床常用抗菌药物耐药和携带耐药基因的情况，以及不同动物源耐药沙门氏菌之间的亲缘关系，从新疆焉耆县几个规模化养殖场分别采集鸡、牛、猪及羊肛拭子样；对样品中分离出的沙门氏菌运用琼脂稀释法进行13种抗菌药物的最小抑菌浓度测定；并对耐药菌株通过PCR方法进行相关耐药基因的检测；采用MLST方法分析不同动物源耐药沙门氏菌之间的亲缘关系。结果显示：①分离鸡源沙门氏菌110株（27.5%；110/400），牛源沙门氏菌63株（64.9%；63/97），猪源沙门氏菌40株（10.0%；40/400），羊源沙门氏菌17株（6.8%；17/250）。②牛源沙门氏菌对氨苄西林、卡那霉素以及氟苯尼考这3种抗菌药物的耐药率均在50.0%以上，以7耐（33.3%）为主；猪源沙门氏菌对四环素的耐药率高于本地区其他动物源菌，多药耐药结果显示在0耐~7耐均有分布。③牛源沙门氏菌和羊源沙门氏菌中blaTEM、blaOXA、oqxA、oqxB、aac(6′)-Ib-cr、ant(3″)-Ia、aadA2和tetB基因的携带率高于鸡源和猪源沙门氏菌的携带率，但blaCMY-2、qnrB、qnrS和tetA这4种耐药基因仅在猪源沙门氏菌中检出。④筛选出8株多药耐药且携带多种耐药基因的沙门氏菌，MLST分析发现8株菌的ST型均为ST34，进化树分析显示它们之间存在较近的亲缘关系。结果表明，焉耆县被检养殖场的动物粪便中均分离出沙门氏菌，且对13种不同抗菌药物表现出不同程度的耐药，存在多种耐药基因共存的情况，不同动物源耐药沙门氏菌间存在克隆传播的机制。
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| 关 键 词： | 沙门氏菌 耐药性 耐药基因检测 MLST分析 |
| 收稿时间： | 2019-03-21 |
MLST Analysis of Salmonella Isolates From Different Animal Sources in Yanqi County of Xinjiang |
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| WANG Kai,LIN Yajun,WANG Shufeng,LI Shuxian,XIANG Zhiyu,XIA Lining.MLST Analysis of Salmonella Isolates From Different Animal Sources in Yanqi County of Xinjiang[J].Journal of Agricultural Science and Technology,2020,22(6):111-122. |
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| Authors: | WANG Kai LIN Yajun WANG Shufeng LI Shuxian XIANG Zhiyu XIA Lining |
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| Institution: | College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | In order to understand the drug resistance of Salmonella spp. from different animal sources to commonly used antibiotics and the distribution of drug-resistant genes in Yanqi County, Xinjiang province, as well as the relationship between drug-resistant Salmonella from different animal sources, anal swabs of chicken, cattle, pig and sheep from several farms in Yanqi County, Xinjiang were sampled, the minimum inhibitory concentration of 13 antimicrobial agents on Salmonella isolated from samples were performed using agar dilution method,and PCR was used to detect the drug-resistant strains. Then MLST method was used to analyze the relationship of Salmonella from different animals. Results showed that: ① 110 strains of Salmonella were isolated from chicken (27.5%, 110/400), 63 strains of Salmonella were isolated from cattle (64.9%, 63/97) and 40 strains of Salmonella were isolated from swine (10.0% 40/400), 17 strains of Salmonella were isolated from sheep (6.8%, 17/250). ② The resistance rate of Salmonella isolates from cattle to ampicillin, kanamycin and florfenicol was above 50.0%, and the resistance to 7 antibiotics was the main trend, the percentage was 33.3%. The resistance rate of Salmonella from pigs to tetracycline was higher than that from other animals in tested region, the isolates showed multiple-drug resistance with resistance to 0~7 multi-drug. ③ Carrying rate of blaTEM, blaOXA, oqxA, oqxB, aac(6′)-Ib-cr, ant(3″)-Ia, aadA2 and tetB genes in Salmonella from cattle and sheep were higher than those in chicken and pig. However, blaCMY-2, qnrB, qnrS and tetA genes were detected only in Salmonella from pig. ④ MLST analysis suggested that 8 strains of Salmonella from different animal sources were all ST34. Phylogenetic tree analysis suggested that there was a close genetic relationship between them. It was suggested that Salmonella were isolated from all animal feces in the farms investigated in Yanqi County. There was difference of resistance to the tested antibiotics between Salmonella isolates. The multi-drug resistance genes were coexistent, and clonal transmission existed among resistant Salmonella strains from different animal sources. |
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| Keywords: | Salmonella drug resistance detection of drug resistance genes MLST analysis |
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